To validate the SlNAC2 transcript level in wild-type, pBI121 plants, and overexpression transgenic lines of SlNAC2, the Plant Total RNA Purification Kit (Tiangen, Beijing, China) was used to extract total RNA from 7-day-old seedlings. Then, using the reverse transcription kit (TransGen Biotech, Beijing, China), 1.5 ug RNA was reverse-transcribed into cDNA. The AtACTIN2 (NM_112764) was used as an internal reference gene. RT-PCR primers are presented in Table S9 .
To validate the DEGs using RT-qPCR, 15 genes responding to salt stress were selected from the RNA-seq studies. Total RNA samples from SlD, SlG_C, and SlG_N were extracted as stated in the Plant Total RNA Purification Kit (Tiangen, Beijing, China). An amount of 1.5 ug RNA was converted into cDNA using the reverse transcription kit (TransGen Biotech, Beijing, China). RT-qPCR experiments were conducted using fluorescence quantitative PCR kit (TransGen Biotech, Beijing, China), and then analyzed via the Real-Time PCR machine (Takara, Japan). The cycle threshold (Ct) 2−ΔΔCt method was used to calculate the relative expression levels of these genes. The SlACTIN (GenBank No. JX860282) was used as an internal reference gene. The primers for RT-qPCR are presented inTable S9 .
To validate the DEGs using RT-qPCR, 15 genes responding to salt stress were selected from the RNA-seq studies. Total RNA samples from SlD, SlG_C, and SlG_N were extracted as stated in the Plant Total RNA Purification Kit (Tiangen, Beijing, China). An amount of 1.5 ug RNA was converted into cDNA using the reverse transcription kit (TransGen Biotech, Beijing, China). RT-qPCR experiments were conducted using fluorescence quantitative PCR kit (TransGen Biotech, Beijing, China), and then analyzed via the Real-Time PCR machine (Takara, Japan). The cycle threshold (Ct) 2−ΔΔCt method was used to calculate the relative expression levels of these genes. The SlACTIN (GenBank No. JX860282) was used as an internal reference gene. The primers for RT-qPCR are presented in