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Human st2 il 33 r quantikine elisa kit

Manufactured by R&D Systems
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The Human ST2/IL-33 R Quantikine ELISA Kit is a quantitative sandwich enzyme immunoassay designed to measure human ST2/IL-33 R levels in cell culture supernates, serum, and plasma.

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Lab products found in correlation

Human calprotectin, by Hycult Biotech (1 mentions) Elecsys 2010, by Roche (1 mentions) Nf light simoa assay advantage kit, by Quanterix (1 mentions) P tau181 advantage v2 kit, by Quanterix (1 mentions)

Close spelling variants of this product

Human IL-33 Quantikine ELISA Kit Quantikine Human IL-33 Human IL-33 Quantikine ELISA kit IL-33 Quantikine ELISA Quantikine Human Quantikine kits Quantikines Human kits

5 protocols using human st2 il 33 r quantikine elisa kit

1

Serum and Fecal Biomarkers for Intestinal Health

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Serum concentration of Intestinal Fatty Acids-Binding Protein (I-FABP) and the soluble IL-33 receptor sST2 were measured by ELISA (Human Intestinal Fatty Acid Binding Protein I-FABP ELISA kit; CUSABIO, Houston, TX, USA and Human ST2/IL-33R Quantikine ELISA kit, R&D Systems, Minneapolis, MN, USA), following each manufacturer protocols. Also, fecal levels of secretory IgA (sIgA), calprotectin and lactoferrin were determined by ELISA, employing the following kit: Secretory IgA ELISA, sIgA (ALPCO, Salem, NH, USA), Human Lactoferrin and Human Calprotectin (both Hycult Biotech, Uden, the Netherlands) according to manufacturer instructions.
Ruiz-Briseño M.D., De Arcos-Jiménez J.C., Ratkovich-González S., Sánchez-Reyes K., González-Hernández L.A., Andrade-Villanueva J.F, & Alvarez-Zavala M. (2020). Association of intestinal and systemic inflammatory biomarkers with immune reconstitution in HIV+ patients on ART. Journal of Inflammation (London, England), 17, 32.
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2

Vitamin D, sST2, and PTH Measurements

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Blood samples were taken between 07:00 and 11:00 h in the morning after an overnight fast at both study visits. The samples were either measured by routine on-site laboratory procedures or stored at −80°C until analysis. Measurements of serum 25(OH)D were performed by means of a chemiluminescence assay (IDS-iSYS 25-hydroxyvitamin D S assay; Immunodiagnostic Systems Ltd., Boldon, UK) on an IDS-iSYS multidiscipline automated analyser with an intra-assay and inter-assay coefficient of variation (CV) of 6.2 and 11.6%, respectively. sST2 was measured in serum with the Human ST2/IL-33 R Quantikine ELISA Kit (R&D Systems). The intra-assay CVs were 4.5–5.6% and inter-assay CVs were 6.3–7.1%, while the lower limit of detection was 5.1 pg/mL according to the manufacturer. PTH was measured using a sandwich ElectroChemiLuminescence Immunoassay (ECLIA) on an Elecsys 2010 immunoassay analyser (Roche Diagnostics), with inter-assay CVs 5.7–6.3%. Measurements of other parameters have been described previously (23 (link)).
Francic V., Keppel M., Schwetz V., Trummer C., Pandis M., Borzan V., Grübler M.R., Verheyen N.D., Kleber M.E., Delgado G., Moissl A.P., Dieplinger B., März W., Tomaschitz A., Pilz S, & Obermayer-Pietsch B. (2019). Are soluble ST2 levels influenced by vitamin D and/or the seasons?. Endocrine Connections, 8(6), 691-700.
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3

Serum sST2 Levels in Hemodialysis Patients

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The patients’ medical history, demographic characteristics, medications and dialytic prescriptions were collected during the recruitment period. Predialysis biochemical indicators (e.g., hemoglobin (Hb), albumin (ALB), creatinine (SCr), blood urea nitrogen (BUN), serum iron (SI), serum ferritin (SF), C-reactive protein (CRP) and NT-proBNP) and postdialysis BUN, were measured in the central clinical laboratory of Ruijin Hospital and collected during the recruitment period.
The remaining predialysis blood serum samples were preserved at −80 °C for further detection. Patients’ serum sST2 levels were detected by Human ST2/IL-33R Quantikine ELISA Kit (R&D Systems, Minneapolis, MN). Each sample was tested twice in twin wells to calculate the mean value as the final level of serum sST2. The detection range of the serum sST2 immunoassay was 6.74–20.42 ng/ml. The intra-assay precision was 4.4–5.6%, and the interassay precision was 5.4–7.1%. No significant cross-reactivity or interference was observed. The formulas for body mass index (BMI), urea reduction ratio (URR), and Kt/V are listed in the Supplementary Material.
Wang Z., Chen Z., Yu H., Ma X., Zhang C., Qu B., Zhang W, & Chen X. (2020). Superior prognostic value of soluble suppression of tumorigenicity 2 for the short-term mortality of maintenance hemodialysis patients compared with NT-proBNP: a prospective cohort study. Renal Failure, 42(1), 523-530.
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4

Biomarkers of Alzheimer's Disease in Chinese and UK Cohorts

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We measured the plasma level of sST2 in 613 individuals from Chinese_cohort_1 (n = 277 individuals with AD, n = 336 HCs); CSF level of sST2 in 86 individuals from the UKBBN cohort (n = 75 individuals with AD, n = 11 HCs); and the level of sST2 secreted by hCMEC/D3 cells using the Human ST2/IL-33 R Quantikine ELISA Kit (DST200; R&D Systems). The plasma levels of NfL (n = 154 individuals with AD, n = 135 HCs) and P-tau181 (n = 156 individuals with AD, n = 134 HCs) in individuals from Chinese_cohort_1 were measured by the Quanterix Accelerator Laboratory using the Quanterix NF-light SIMOA Assay Advantage Kit (103186) and P-Tau 181 Advantage V2 Kit (103714), respectively93 .
Jiang Y., Zhou X., Wong H.Y., Ouyang L., Ip F.C., Chau V.M., Lau S.F., Wu W., Wong D.Y., Seo H., Fu W.Y., Lai N.C., Chen Y., Chen Y., Tong E.P., Mok V.C., Kwok T.C., Mok K.Y., Shoai M., Lehallier B., Losada P.M., O’Brien E., Porter T., Laws S.M., Hardy J., Wyss-Coray T., Masters C.L., Fu A.K, & Ip N.Y. (2022). An IL1RL1 genetic variant lowers soluble ST2 levels and the risk effects of APOE-ε4 in female patients with Alzheimer’s disease. Nature Aging, 2(7), 616-634.
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5

Quantification of Soluble ST2 by ELISA

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The quantification of sST2 was performed by using a commercially available kit: Human ST2/IL-33R Quantikine ELISA Kit (DST 200, R&D). The assay detects free and IL-33-complexed ST2. Analyses were performed in duplicates according to the manufacturer’s instructions. Sample predilution was adjusted individually to the concentrations. The range of assay sensitivity was 2.45-13.5 pg/mL. Reference blood samples from healthy adults were used for comparison.
Erfurt S., Hoffmeister M., Oess S., Asmus K., Patschan S., Ritter O, & Patschan D. (2022). Soluble IL-33 receptor predicts survival in acute kidney injury. Journal of Circulating Biomarkers, 11, 28-35.
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