Log In Sign up
The largest database of trusted experimental protocols

Fc blocking antibody

Manufactured by BD
Visit Supplier
Sourced in United States

The Fc blocking antibody is a laboratory reagent designed to prevent non-specific binding of antibodies to Fc receptors. It functions by binding to the Fc portion of antibodies, effectively blocking their interaction with Fc receptors on cells or other surfaces. This product is commonly used in immunoassays, cell-based assays, and other experimental procedures where Fc-mediated interference needs to be minimized.

Automatically generated - may contain errors

Lab products found in correlation

Facscelesta, by BD (2 mentions) Flow cytometry staining buffer, by BD (2 mentions) Fixable viability stain, by BD (2 mentions) Horizon brilliant stain buffer, by BD (2 mentions) Facscanto 2 flow cytometer, by BD (1 mentions) Anti cd86 pe cy5 clone gl1, by Thermo Fisher Scientific (1 mentions) Mitotracker green fm, by Thermo Fisher Scientific (1 mentions) Mitosox red mitochondrial superoxide indicator, by Thermo Fisher Scientific (1 mentions) Mitotracker red cmxros, by Thermo Fisher Scientific (1 mentions) Ultra low attachment plate, by Corning (1 mentions) Live dead fixable dead cell stain kit, by Thermo Fisher Scientific (1 mentions) E50 2440, by BD (1 mentions) Clone 2.4g2, by BD (1 mentions) Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions) F4 80 pe, by BD (1 mentions) Percp cy5.5 cd11c, by BD (1 mentions) Cd11b fitc, by BD (1 mentions) Cd19 apc, by BD (1 mentions) Cd3 pe, by BD (1 mentions) Facscanto 2, by BD (1 mentions)

Close spelling variants of this product

Fc receptor blocking antibody (6 citations) Fc blocking anti-mouse CD 16/32 antibody (3 citations) Fc-blocking antibody 2.4 G2 (2 citations) Anti-CD16/CD32 Fc blocking antibody (clone 2.4G2) (2 citations) Fc-gamma blocking antibody (2.4G2, (2 citations)

12 protocols using fc blocking antibody

1

Macrophage Immunophenotyping by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peritoneal macrophages were incubated with an Fc blocking antibody (Clone 2.4G2, BD Biosciences) for 20 min at 4°C and then stained with anti-CD11b-PE, anti-F4/80-FITC antibodies for 30 min at 4°C. Intracellular staining was performed, if necessary, with anti-CD206-APC or anti-iNOS-APC antibody. Data were acquired on a FACSCanto II flow cytometer (BD Biosciences, San Jose, CA, USA) and analyzed by using FlowJo (Tree Star, Inc., Ashland, OR, USA).
Wang L., Gong Z., Zhang X., Zhu F., Liu Y., Jin C., Du X., Xu C., Chen Y., Cai W., Tian C, & Wu J. (2020). Gut microbial bile acid metabolite skews macrophage polarization and contributes to high-fat diet-induced colonic inflammation. Gut Microbes, 12(1), 1819155.
+ Open protocol
+ Expand
2

Multiparametric Phenotypic Profiling of Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were plated in ultra-low attachment plates (Corning) and cultured
in basal DMEM, pH 6.8 or 7.4, for 24 h. Cells were then treated with Fc blocking
antibody (purified anti-mouse CD16/32, BD) for 15 mins and stained with
anti-F4/80-BV421 (clone BM8, Biolegend), anti-CD206-APC (clone C068C2,
Biolegend), anti-CD301(Mgl1)-PE (clone LOM-14, Biolegend), anti-MHC II-PE-Cy7
(clone M5/114.15.2, Biolegend) and anti-CD86-PE-Cy5 (clone GL1, eBioscience)
antibodies for 30 min on ice in dark. To analyze mitochondria, live cells were
stained with MitoTracker™ Green FM,
MitoTracker™ Red CMXRos and MitoSOX™ Red
Mitochondrial Superoxide Indicator (Invitrogen) to determine mitochondrial mass,
inner membrane potential and reactive oxygen species (ROS) respectively. After
wash, cell fluorescence was measured using flow cytometry (BD FACSCelesta) and
the data were analyzed using Flow Jo software (TreeStar, Inc).
Jiang W., Le J., Wang P.Y., Cheng X., Smelkinson M., Dong W., Yang C., Chu Y., Hwang P.M., Munford R.S, & Lu M. (2021). Extracellular acidity reprograms macrophage metabolism and innate responsiveness. Journal of immunology (Baltimore, Md. : 1950), 206(12), 3021-3031.
+ Open protocol
+ Expand
3

Single-cell Immune Profiling by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
The single-cell suspensions from above were pelleted, aliquoted at ~1 × 106 cells per tube, and stained with Fc blocking antibody (5 μg/ml, BD) and Live/Dead Fixable Dead Cell Stain Kit (Invitrogen) at room temperature for 10 min. The cells were washed and then incubated with surface marker antibody cocktail for 45 min at 4 °C. For sorting ILC2s and Th2 cells, the following antibodies were used (all antibodies are from Biolegend, used at 2 μg/ml, unless otherwise specified): BV785-conjugated anti-CD90.2 (30/H12), AF700-conjugated anti-B220 (RA36B2), AF700-conjugated anti-CD11b (M1/70), AF700-conjugated anti-CD11c (N418), AF700-conjugated anti-Nk1.1 (PK136), BV510-conjugated anti-CD4 (GK1.5), PE-Cy7-conjugated anti-TCR beta (H57-597), FITC-conjugated anti-TCR delta (GL3), and PE-conjugated anti-ST2 (DIH9). For sorting eosinophils, the following antibodies were used: PE-conjugated anti-CD64 (X54-5/7.1), PE-Cy7-conjugated anti-CD11c (N418), and BB515 anti-Siglec F (2 μg/ml, E50-2440, BD). Samples were analyzed on an LSR II (BD Biosciences) with four lasers (405 nm, 488 nm, 561 nm, and 635 nm). Data were analyzed with FlowJo software (Treestar).
Sui P., Wiesner D.L., Xu J., Zhang Y., Lee J., Van Dyken S., lashua A., Yu C., Klein B.S., Locksley R.M., Deutsch G, & Sun X. (2018). Pulmonary Neuroendocrine Cells Amplify Allergic Asthma Responses. Science (New York, N.Y.), 360(6393), eaan8546.
+ Open protocol
+ Expand
4

Corneal Graft Immune Cell Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Grafted corneas were harvested two weeks post-transplantation and incubated in 20 mM EDTA (Sigma-Aldrich Inc., St. Louis, MO) at 37°C for 45 minutes to facilitate the removal of the epithelium. Subsequently, tissue was fixed in ice-cold acetone for 20 minutes, washed, and blocked with 3% BSA for 60 minutes. Next, corneas were incubated with Fc-blocking antibody (1:100, clone 2.4G2; BD PharMingen) and then with FITC-CD103 and 594-CD11c antibodies (Table 1) at 4°C overnight. Then, corneas were imaged using a TCS SP8 multiphoton microscope (Leica Microsystems Inc., Morrisville, NC), and images were processed using ImageJ software (National Institutes of Health, Bethesda, MD).
Blanco T., Singh R.B., Nakagawa H., Taketani Y., Dohlman T.H., Chen Y., Chauhan S.K., Yin J, & Dana R. (2023). Conventional Type I Migratory CD103+ Dendritic Cells are Required for Corneal Allograft Survival. Mucosal immunology, 16(5), 711-726.
+ Open protocol
+ Expand
5

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single-spleen cell suspensions and draining lymph node cell suspensions were obtained from mice one week after the last immunization. Cells (1×106/mL) were blocked with Fc blocking antibody (BD Bioscience) in fluorescence-activated cell sorting (FACS) buffer (phosphate buffered saline, 1.0% fetal bovine serum) for 15 min at 4°C. The cells were washed and stained with fluorochrome-conjugated antibodies against lymphocyte surface receptors, APC-Mouse I-Ab, PerCP-Cy5.5-CD11c, PE-F4/80, FITC-CD11b, PE-CD3 and APC-CD19 (BD Biosciences) for 30 min at 4°C. The cells were then washed and fixed with 2% paraformaldehyde solution for 20 min at 4°C. Data were acquired on a BD FACS Canto II flow cytometer (BD Bioscience) with at least 1 × 105 events for each sample and analysed using FlowJo software (Tree Star Inc, Ashland, OR, USA).
Du X., Xue J., Jiang M., Lin S., Huang Y., Deng K., Shu L., Xu H., Li Z., Yao J., Chen S., Shen Z, & Feng G. (2021). A Multiepitope Peptide, rOmp22, Encapsulated in Chitosan-PLGA Nanoparticles as a Candidate Vaccine Against Acinetobacter baumannii Infection. International Journal of Nanomedicine, 16, 1819-1836.
+ Open protocol
+ Expand
6

Carnoy's Fixation and Immunofluorescence Staining of Mouse Colon

Check if the same lab product or an alternative is used in the 5 most similar protocols
For Carnoy’s fixation, 1 cm of colon was dissected from the mouse and unfolded into a square shape. The tissues were fixed using Carnoy’s fixative solution (10% glacial acetic acid, 30% chloroform, 60% ethanol, and 1 g ferric chloride) and embedded in frozen section medium (Leica Biosystems, Nussloch, Germany) at −80 °C for 1 h before storage at –20 °C. For further staining, we sectioned blocks into 6 μm using a cryotome (Leica Biosystems), fixed them in precooled (−20 °C) acetone for 2 min, and washed them in fresh PBS for 5 min. The tissues were incubated with Fc blocking antibody (BD Biosciences, San Jose, CA, USA) for 30 min at room temperature and then with anti-Muc2 antibody (Abcam, Cambridge, UK) for 2 h at room temperature as well. After rinsing in fresh PBS, the tissues were incubated with Alexa Fluor 488-conjugated anti-IgG antibody (Abcam), counterstained with NucRed Live 647 ReadyProbes Reagent (Invitrogen, Carlsbad, CA, USA), and observed under a Leica DMi8 microscope (Leica Microsystems, Wetzlar, Germany).
Lee S.H., Seo D., Lee K.H., Park S.J., Park S., Kim H., Kim T., Joo I.H., Park J.M., Kang Y.H., Lim G.H., Kim D.H, & Yang J.Y. (2023). Biometabolites of Citrus unshiu Peel Enhance Intestinal Permeability and Alter Gut Commensal Bacteria. Nutrients, 15(2), 319.
+ Open protocol
+ Expand
7

Uptake of OVA peptide by mouse dendritic cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
DC2.4 mouse dendritic cells were seeded overnight in a 12 well plate at 1 × 106 cells/mL (1 mL per well) in complete RPMI media. The next day, 500 μL of media was aspirated and 500 μL of TAMRA-pOVA or media were added to pOVA-treated and untreated wells, respectively. For the tablet group, 500 μL of media was added to each well and the tablets were gently dropped into the wells to dissolve. All groups contained 20 nmol of total peptide per well. After incubation for 2 or 6 hours, the cells were prepared for flow cytometry. Cells were treated with Fc blocking antibody (BD Biosciences, cat # 553141) for 30 min and stained with CD11c:PE-Cy7 (BD Biosciences, cat #561022) for 30 min. Flow cytometry was performed on a FACS Canto cytometer and data was analyzed using FlowJo software.
Kelly S.H., Opolot E.E., Wu Y., Cossette B., Varadhan A.K, & Collier J.H. (2021). Tabletized Supramolecular Assemblies for Sublingual Peptide Immunization. Advanced healthcare materials, 10(6), e2001614.
+ Open protocol
+ Expand
8

Dendritic Cell Uptake of Microparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols
All animal studies were carried out under the supervision of the University of Maryland Institutional Animal Care and Use Committee (IACUC) in compliance with local, state, and federal guidelines. To test dendritic cell (DC) uptake of MPs, CD11c+ DCs were isolated from the spleens of female non-obese diabetic (NOD/ShiLtJ) mice (Jackson Laboratories) using CD11c positive selection following manufacturer’s instructions (Milltenyi). DCs were plated at 100,000 cells/well in RPMI1640+L-glutamine Media (Thermo Fisher) supplemented with 10% fetal bovine serum (Corning), 2 mM L-glutamine (Gibco), 55 μM β-mercaptoethanol (Sigma-Aldrich) 1 X Non-Essential Amino Acids (Fisher Scientific), 10 mM HEPES (Fisher Scientific), and 1 X Pen/Strep (Gibco). Lipopolysaccharide (LPS) (Sigma) was added to 1 μg/ml and DiO-labeled MP formulations were added. After 24 hours, cells were stained with FC blocking antibody (BD), then stained with anti-CD11c (BD) and resuspended in 4′,6-Diamidino-2-Phenylindole, Dihydrochloride (DAPI). Flow cytometry was performed on either BD FACSCelesta (BD) or BC Cytoflex (BC) to assess MP internalization. All flow cytometric analysis was performed using FlowJo.
Carey S.T., Gammon J.M, & Jewell C.M. (2021). Biomaterial-enabled induction of pancreatic-specific regulatory T cells through distinct signal transduction pathways. Drug delivery and translational research, 11(6), 2468-2481.
+ Open protocol
+ Expand
9

Flow Cytometric Analysis of TNFR1 and IL1R1

Check if the same lab product or an alternative is used in the 5 most similar protocols
Confluent FLS cells were washed once with PBS and harvested by incubating the monolayer in 10 ml PBS containing 0.02% EDTA and detaching cells by firmly rocking. Unspecific staining was reduced by incubating the re-suspended cells in HBSS containing 1% BSA, 2% bovine FCS and 0.1% NaN 3 in the presence of 0.2 µl/well Fc blocking antibody (BD Biosciences) for 10 min on ice. For TNFR1 staining, 40 µl cell suspension in HBSS plus supplements were incubated with anti-TNFRI antibody (Abcam) or the equal concentration of isotype control rabbit IgG (R&D systems.) A secondary anti-rabbit A488 antibody (Life Technologies) was used. For IL1R1 staining the anti-IL1R1 PE antibody (BD Biosciences) or the respective isotype control (BD Biosciences) was used. For both stainings, dead cells were excluded by incubating with DAPI (Life Technologies) before recording on a FACS Canto II cytometer.
Godmann L., Bollmann M., Korb-Pap A., König U., Sherwood J., Beckmann D., Mühlenberg K., Echtermeyer F., Whiteford J., De Rossi G., Pap T, & Bertrand J. (2020). Antibody-mediated inhibition of syndecan-4 dimerisation reduces interleukin (IL)-1 receptor trafficking and signalling. Annals of the rheumatic diseases, 79(4).
+ Open protocol
+ Expand
10

Immune Cell Phenotyping by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
IHL and splenocytes isolated were stained with monoclonal antibodies after being treated with FC blocking antibody (BD, Franklin Lakes, NJ). Antibodies against mouse IFN-γ, CD4, CD8, CD3, c-Met, MHC-II, CD40, CD86, CD44, CD62L, PD-1 andTim-3 were purchased from eBioscience (San Diego, CA). Data were acquired with the LSR II Fortessa system (BD Biosciences) and analyzed with FlowJo 8.5 software (TreeStar, Ashland, OR).
Aguilar-Valenzuela R., Carlsen E.D., Liang Y., Soong L, & Sun J. (2014). Hepatocyte Growth Factor Dampens Liver Immune-Mediated Pathology in Acute Viral Hepatitis without Compromising Antiviral Activity. Journal of gastroenterology and hepatology, 29(4), 878-886.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!