Crl 2242

Human PC cell lines (PC346, LNCap, MDAPC1 2a/b, C4-2, PC3, and DU145) were obtained from the American Type Culture Collection (Catalog number CRL-2876, CRL-1740, CRL-2242, CRL-3314, CRL-1435, and HTB-81, respectively, ATCC, Rockville, MD, USA). Human PC cell line (BPH1) was obtained from Accegen Biotechnology (Catalog number ABC-TC454S, Fairfield, NJ, USA). All lines were maintained in DMEM (Invitrogen, Rockville, MD, USA) suppled with 5% fetal bovine serum (FBS; Sigma-Aldrich, Rockville, MD, USA) in a humidified incubator at 37 °C with a 5% CO₂ atmosphere.

Human cervical HeLa cells [CCL-2; American Type Culture Collection (ATCC), Manassas, VA, USA] and HEK293 cells (CRL-1573; ATCC) were cultured in DMEM (Sigma-Aldrich) that was supplemented with 10% fetal bovine serum (FBS; GE Healthcare Life Sciences), 4 mM l-glutamine, and 1% penicillin/streptomycin (all from Sigma-Aldrich) in 5% CO₂ at 37°C. CHO-K1 cells (CCL-61; ATCC) and mutant pgsA-745 cells (CRL-2242; ATCC) were cultured in Ham’s Nutrient Mixture F12 (Sigma-Aldrich) that was supplemented with 10% FBS, 4 mM l-glutamine, and 1% penicillin/streptomycin in 5% CO₂ at 37°C. Once cells reached ∼95% confluence, they were split by using 0.25% trypsin-EDTA (Sigma-Aldrich) into fractions and propagated or used in experiments.