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3 protocols using sinensetin

1

Tetramethylated Flavonoid Compounds

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Nobiletin, sinensetin, tangeretin, and scutellarein tetramethylether were purchased from Extrasynthese (Lyon, France). Stock solutions were prepared in DMSO (Sigma-Aldrich, St. Louis, MO, USA), and stored at 4 °C.
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2

Identification and Quantification of Citrus Flavonoids

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HPLC-grade acetonitrile and DMSO were obtained by Sigma-Aldrich (St. Louis, MO, USA). Vicenin-2, hesperidin, eriocitrin, narirutin, sinensetin, nobiletin, and tangeretin were supplied from Extrasynthèse (Genay, France). Lucenin-2 4′-methylether and orientin 4′-methyl ether were separated from Citrus limetta and Citrus bergamia [14 (link),15 (link)] and used as standards. The Iso-Disc P-34, 3 mm diameter PTFE membrane (0.45 μm pore size) was from Supelco (Bellefonte, PA, USA). All the other reagents and chemicals employed in this study were of analytical grade and were purchased from Sigma (St. Louis, MO, USA).
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3

Quantitative Analysis of Flavonoids and Synephrine

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Naringin (naringenin 7-O-neohesperidoside, 8), hesperidin (hesperetin 7-O-rutinoside, 9), sinensetin (3′,4′,5,6,7-pentamethoxyflavone, 12), and synephrine were purchased from Extrasynthèse and used as standards for qualitative and quantitative analyses. Their purity was higher than 98 %.
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