Pas haematoxylin stain kit

Retinal PAS staining is a method used to detect the amounts of acellular capillaries in the retina. The retinas were fixed in 4% paraformaldehyde for 1.5 h and digested in 3% trypsin for 2 h at 37 °C. The samples were gently washed until the retinal leaves were completely spread. Visualized vessels were dried on carrier slides and then retinal capillaries were dyed with the PAS/haematoxylin stain kit (Solarbio, Beijing, China), following the manufacturer’s instructions. The samples were assessed under a light microscope (20×, Olympus, Tokyo, Japan).

The amount of acellular capillaries was determined by retinal PAS staining according to the procedure previously described (Laver et al., 1993 (link); Huang et al., 2015 (link)). The retina of rats was Briefly, the enucleated eyes were fixed with 4% paraformaldehyde for 24 h. Flat retinas were successfully isolated and fixed in 4% paraformaldehyde for 1.5 h and then digested with 3% trypsin for 2 h and samples were stained Nonvascular tissues were gently flushed away by a 1 ml syringe filled with PBS, and the visible vessels were mounted on glass slides under the microscope. After drying overnight, the samples were stained using the PAS/haematoxylin stain kit in accordance with the instructions (Solarbio, China). Degeneration of retinal capillaries can be was observed under light microscopy (Olympus, Tokyo, Japan).