Chromium single cell 3 v2 reagent

Manufactured by 10x Genomics

The Chromium Single Cell 3' v2 Reagent is a lab equipment product offered by 10x Genomics. It is designed to enable single-cell RNA sequencing analysis.

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Lab products found in correlation

Chromium single cell 5 d j reagent kit, by 10x Genomics (1 mentions) Gel bead and multiplex kit, by 10x Genomics (1 mentions) Chromium single cell 5 v2 reagent, by 10x Genomics (1 mentions) Chromium single cell 3 v 3 reagent, by 10x Genomics (1 mentions) Chromium single cell 5 library, by 10x Genomics (1 mentions) Chip kit, by 10x Genomics (1 mentions) Chromium single cell 3 library, by 10x Genomics (1 mentions)

Close spelling variants of this product

Chromium Single Cell 3′ v2 (4 citations)

2 protocols using chromium single cell 3 v2 reagent

Single-Cell RNA Sequencing of Pituitary Cells

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Dissociated pituitary cells were diluted at 500 cells per µl and processed using Chromium Single Cell 3′ v2 Reagent (10x Genomics, Pleasanton, CA) following the manufacturer recommendation. Briefly, cells were passed on the channel and 9269 cells were recovered. Pituitary cells were partitioned into gel beads in emulsion for cell lysis and barcoded with oligo-dT priming and reverse transcribed. cDNA library was amplified fragmented and size selected. Samples were controlled at multiple steps during the procedure by running on BioAnalyzer. Libraries were sequenced on Hiseq 4000 with 100 bp paired-end reads.

Mayran A., Sochodolsky K., Khetchoumian K., Harris J., Gauthier Y., Bemmo A., Balsalobre A, & Drouin J. (2019). Pioneer and nonpioneer factor cooperation drives lineage specific chromatin opening. Nature Communications, 10, 3807.

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Single-cell RNA Sequencing of Cell Suspensions

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Cell suspensions were barcoded through the 10x Chromium Single Cell platform using Chromium Single Cell 5′ Library, Chromium Single Cell 3′ Library, Gel Bead and Multiplex Kit, and Chip Kit (10x Genomics). The loaded cell numbers range from 300-500,000 aiming for 300-14,000 single cells per reaction. Single-cell RNA libraries were prepared using the Chromium Single Cell 3′ v2 Reagent (10x Genomics; PN-120237, PN-120236 and PN-120262), Chromium Single Cell 3′ v3 Reagent (10x Genomics; PN-1000075, PN-1000073 and PN-120262) the Chromium Single Cell 5′ v2 Reagent (10x Genomics, 120237), and Chromium Single Cell V(D)J Reagent kits (10x Genomics, PN-1000006, PN-1000014, PN-1000020, PN-1000005) was used to prepare single-cell RNA libraries according to the manufacturer’s instructions. Each sequencing library was generated with a unique sample index. The libraries were sequenced using either DIPSEQ, BGISEQ or Illumina platforms.

Ren X., Wen W., Fan X., Hou W., Su B., Cai P., Li J., Liu Y., Tang F., Zhang F., Yang Y., He J., Ma W., He J., Wang P., Cao Q., Chen F., Chen Y., Cheng X., Deng G., Deng X., Ding W., Feng Y., Gan R., Guo C., Guo W., He S., Jiang C., Liang J., Li Y.M., Lin J., Ling Y., Liu H., Liu J., Liu N., Liu S.Q., Luo M., Ma Q., Song Q., Sun W., Wang G., Wang F., Wang Y., Wen X., Wu Q., Xu G., Xie X., Xiong X., Xing X., Xu H., Yin C., Yu D., Yu K., Yuan J., Zhang B., Zhang P., Zhang T., Zhao J., Zhao P., Zhou J., Zhou W., Zhong S., Zhong X., Zhang S., Zhu L., Zhu P., Zou B., Zou J., Zuo Z., Bai F., Huang X., Zhou P., Jiang Q., Huang Z., Bei J.X., Wei L., Bian X.W., Liu X., Cheng T., Li X., Zhao P., Wang F.S., Wang H., Su B., Zhang Z., Qu K., Wang X., Chen J., Jin R, & Zhang Z. (2021). COVID-19 immune features revealed by a large-scale single-cell transcriptome atlas. Cell, 184(7), 1895-1913.e19.

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