Modified dna oligonucleotides

Manufactured by Integrated DNA Technologies

Modified DNA oligonucleotides are synthetic DNA sequences with custom modifications. They are used as tools for a variety of applications in molecular biology and genetics research.

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Lab products found in correlation

Staple strands, by Integrated DNA Technologies (1 mentions) Trastuzumab, by Roche (1 mentions) Cetuximab, by Merck Group (1 mentions) Anti tom20 antibody sc 11415, by Santa Cruz Biotechnology (1 mentions) Cos 7 cells, by Korean Cell Line Bank (1 mentions) Donkey anti rat igg antibody, by Jackson ImmunoResearch (1 mentions) Anti tubulin antibody, by Abcam (1 mentions) Carboxyl latex beads, by Thermo Fisher Scientific (1 mentions) Triton x 100, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Glutaraldehyde, by Merck Group (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions)

Spelling variants of this product

Oligonucleotides (424 citations) DNA oligonucleotides (166 citations) Thiol-modified DNA oligonucleotides (3 citations) Modified oligonucleotides (2 citations) 5’ amine-modified DNA oligonucleotides (2 citations)

2 protocols using modified dna oligonucleotides

Nanomaterial Cell Labeling Protocol

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Staple
strands, handle-extended staple strands,
modified DNA oligonucleotides, and fluorescently modified DNA oligonucleotides
were obtained from Integrated DNA Technologies. The p7560 scaffold
for the nanorod and M13mp18 scaffold for the rectangle were obtained
from Eurofins. Monoclonal antibodies anti-PD1 (kindly provided by
Aduro Biotech, hPD1.27.C4, batch: 18-FJ8381), Cetuximab (Erbitux,
Merck), and Trastuzumab (Herceptin, Roche) were used for cell labeling.

Cremers G.A., Rosier B.J., Meijs A., Tito N.B., van Duijnhoven S.M., van Eenennaam H., Albertazzi L, & de Greef T.F. (2021). Determinants of Ligand-Functionalized DNA Nanostructure–Cell Interactions. Journal of the American Chemical Society, 143(27), 10131-10142.

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Fluorescently-labeled Antibody Conjugation

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Modified DNA oligonucleotides were purchased from Integrated DNA Technologies. Alexa488 (Alexa Fluor 488 NHS Ester, catalog number: A20000) was purchased from Thermo Fisher Scientific. Cy3 (Cy3 NHS Ester, catalog number: PA13101) and Cy5 (Cy5 NHS Ester, catalog number: PA15101) were purchased from GE Healthcare Life Sciences. COS-7 cells were purchased from Korean Cell Line Bank. Anti-tubulin antibody (catalog number: ab6160) was purchased from Abcam. Anti-Tom20 antibody (sc-11415) was purchased from Santa Cruz Biotechnology, Inc. Donkey anti-rabbit IgG antibody (catalog number: 711-005-152) and donkey anti-rat IgG antibody (catalog number: 712-005-153) were purchased from Jackson ImmunoResearch Laboratories, Inc. Carboxyl latex beads (catalog number: C37281) were purchased from Thermo Fisher Scientific. The docking strands were conjugated to the secondary antibodies using Antibody-Oligonucleotide All-in-One Conjugation Kit (catalog number: A-9202-001) purchased from Solulink. Paraformaldehyde (catalog number: 1.04005.1000) was purchased from Merck. Glutaraldehyde (catalog number: G5882), Triton X-100 (catalog number: T9284), and Bovine Serum Albumin (catalog number: A4919) were purchased from Sigma-Aldrich.

Lee J., Park S., Kang W, & Hohng S. (2017). Accelerated super-resolution imaging with FRET-PAINT. Molecular Brain, 10, 63.

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