Sodium thiosulfate

Manufactured by Carl Roth

Sourced in Germany

Sodium thiosulfate is a chemical compound with the chemical formula Na2S2O3. It is a colorless, crystalline solid that is highly soluble in water. Sodium thiosulfate is commonly used as a reducing agent, photographic fixer, and in various chemical reactions and analyses.

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Lab products found in correlation

4 protocols using sodium thiosulfate

Production of Lactozyme-Fortified Cheese Whey

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All chemicals were reagent grade purchased from Merck KGaA (Darmstadt, Germany) except for lecithin, sodium thiosulfate and l-histidine from Carl Roth GmbH (Karlsruhe, Germany), and hydroxypropyl cellulose (Klucel) from Ashland Inc. (Covington, USA). β-galactosidase (Lactozyme 2600 L) was from Novozymes (Bagsvaerd, Denmark), whereas cellulase (Trichoderma reesei) from Merck. Biolife Italiana Srl (Milan, Italy) supplied all culture media ingredients and Agar Listeria Ottaviani Agosti (ALOA) with the dedicated enrichment and selective supplements; De Man, Rogosa and Sharpe (MRS) Broth was from Scharlau Chemie (Barcelona, Spain), Tryptic Soy Broth (TSB) from Merck. CWP was kindly supplied by Latteria Soresina (Soresina, Italy): a single batch was used throughout all the project (lactose 4.7%, protein 0.01%, dry residue 5.5%, ash 0.6%).

Rollini M., Musatti A., Cavicchioli D., Bussini D., Farris S., Rovera C., Romano D., De Benedetti S, & Barbiroli A. (2020). From cheese whey permeate to Sakacin-A/bacterial cellulose nanocrystal conjugates for antimicrobial food packaging applications: a circular economy case study. Scientific Reports, 10, 21358.

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Rye Grain Metabolite Analysis

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Several 1-kg packages of whole rye grains from German organic cultivation were bought in a retail shop in Stuttgart, Germany. Acetic anhydride (> 99%), anhydrous benzotrifluoride (BTF, > 99%), dimethyl disulfide (> 99%), ethyl acetate (> 99%), pyridine (> 99%, distilled before use) and silica gel 60 were from Sigma-Aldrich (Steinheim, Germany) while methanol, n-hexane (both HPLC grade), cyclohexane (> 99%) and toluene (> 99%) were from Th. Geyer (Renningen, Germany). Iodine (> 99.5%) was from Fluka (Taufkirchen, Germany) and sodium thiosulfate (> 99%) was from Carl Roth (Karlsruhe, Germany). The azeotropic mixture cyclohexane/ethyl acetate (CE) (46:54, w/w) was obtained by distillation of mixture (1:1, v/v). N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) and trimethylchlorosilane (TMCS), 99:1 (v/v), was from Macherey Nagel (Düren, Germany). The internal standard 5α-cholestane (> 98%) was from Acros Organics (Geel, Belgium) and docosanoic acid methyl ester (22:0-ME) was prepared by the methylation protocol of Wendlinger et al. [21 (link)] using 40 mg 22:0 (Fluka, Taufkirchen, Germany) and 4 mL methanol. Helium and hydrogen (5.0 quality) were from Westfalen (Münster, Germany). Demineralized water was produced in-house.

Hammerschick T., Wagner T, & Vetter W. (2020). Countercurrent chromatographic fractionation followed by gas chromatography/mass spectrometry identification of alkylresorcinols in rye. Analytical and Bioanalytical Chemistry, 412(30), 8417-8430.

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Synthesis and Characterization of Hydrogel Scaffolds

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Potassium dihydrogen phosphate and silver nitrate were obtained from Merck (Darmstadt, Germany). Di-sodium hydrogen phosphate, acetone, sodium dodecyl sulfate, tris(hydroxymethyl)aminomethane, acetic acid, sodium carbonate, ammonium persulfate, tetramethylenediamine, sodium chloride, sodium thiosulfate and poly(ethylene glycol) (PEG, M_n = 6000 g mol⁻¹) were obtained from Carl Roth (Karlsruhe, Germany). Uranyl acetate and acrylamide/bis solution, 37.5 : 1 were purchased from Serva (Heidelberg, Germany). Spurr Low Viscosity Embedding Kit was obtained from Polysciences (Washington, USA). Poly(ethylene glycol) diacrylate (PEG-DA, M_n = 700 g mol⁻¹) and formaldehyde were obtained from Sigma-Aldrich (Merck; Darmstadt, Germany). The radical photoinitiator 1-[4-(2-hydroxyethoxy)phenyl]-2-hydroxy-2-methyl-1-propan-1-one (Irgacure 2959) was a kind gift from Bodo Möller Chemie GmbH (Offenbach, Germany). A Micro BCA Protein Assay Kit was obtained from Thermo Scientific (Waltham, USA). All chemicals and reagent were used as received. Stock solutions containing 28 mg Irgacure 2959 in 3.972 g SPP buffer were obtained by gentle heating with a heat gun at 100 °C until all material was dissolved, and subsequent cooling to room temperature. Water was withdrawn from a Barnstead GenPure xCAD water purification system (Thermo Scientific, Waltham, USA).

Southan A., Lang T., Schweikert M., Tovar G.E., Wege C, & Eiben S. (2018). Covalent incorporation of tobacco mosaic virus increases the stiffness of poly(ethylene glycol) diacrylate hydrogels. RSC Advances, 8(9), 4686-4694.

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Synthesis and Characterization of Iron Nanoparticles

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Iron(II) chloride tetrahydrate (FeCl₂⋅4H₂O), hydroxyl ammonium chloride, bovine serum albumin (BSA), and Eppendorf ultrafiltration tubes with a molecular weight cutoff (MWCO) of 3 kDa were purchased from Merck (Darmstadt, Germany). Iron(III) chloride hexahydrate (FeCl₃⋅6H₂O), dialysis tubes (Spectrapor 6, MWCO 8 kDa), ammonium chloride, formic acid, hydrochloric acid 25 %, ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), sodium thiosulfate, silver nitrate, sodium carbonate, and ammonia solution 25 % were supplied by Roth (Karlsruhe, Germany). Propidium iodide (PI), sodium citrate, triton X-100, ninhydrin, MES, HEPES, and poly(acrylic acid-co-maleic acid) solution (Mw = 3000 Da) were purchased from Sigma-Aldrich (St Louis, MO, USA). Ringer’s solution was bought from Baxter Healthcare (Zurich, Switzerland). Annexin V-FITC (AxV), Hoechst 33342 (Hoe), and DiIC1(5) (hexamethylindodicarbocyanine iodide dye [DiI]) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).

Friedrich R.P., Zaloga J., Schreiber E., Tóth I.Y., Tombácz E., Lyer S, & Alexiou C. (2016). Tissue Plasminogen Activator Binding to Superparamagnetic Iron Oxide Nanoparticle—Covalent Versus Adsorptive Approach. Nanoscale Research Letters, 11, 297.

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