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Antibiotic antimycotic cocktail solution

Manufactured by Nacalai Tesque
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Sourced in Japan

Antibiotic-antimycotic cocktail solution is a sterile liquid formulation containing a combination of antibiotics and antimycotics. It is designed for use in cell culture applications to prevent microbial contamination.

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Lab products found in correlation

Hygromycin b, by Nacalai Tesque (2 mentions) L glutamine, by Nacalai Tesque (2 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt reagent, by Merck Group (2 mentions) Fetal bovine serum (fbs), by Equitech-Bio (2 mentions) High glucose dulbecco s modified eagle s medium dmem, by Nacalai Tesque (1 mentions)

2 protocols using antibiotic antimycotic cocktail solution

1

Cell Viability Assay with SN-38

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The following reagents and drugs were purchased from the commercial sources indicated in parentheses: antibiotic-antimycotic cocktail solution, l-glutamine, high-glucose Dulbecco’s modified Eagle medium (DMEM), hygromycin B (Nacalai Tesque, Inc., Kyoto, Japan); fetal bovine serum (FBS) (Equitech-Bio, Inc., Kerrville, TX, USA); and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT reagent) (Sigma-Aldrich Co., St. Louis, MO, USA). SN-38 was generously provided by Yakult Honsha Co., Ltd. (Tokyo, Japan). All other chemicals used were of analytical grade.
Tsukamoto M., Sato S., Satake K., Miyake M, & Nakagawa H. (2017). Quantitative Evaluation of Drug Resistance Profile of Cells Expressing Wild-Type or Genetic Polymorphic Variants of the Human ABC Transporter ABCC4. International Journal of Molecular Sciences, 18(7), 1435.
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2

Cytotoxicity Assay Protocol with SN-38

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following reagents and drugs were purchased from the commercial sources indicated in parentheses: antibiotic-antimycotic cocktail solution, l-glutamine, high-glucose Dulbecco’s modified Eagle’s medium (DMEM), hygromycin B (Nacalai Tesque, Inc., Kyoto, Japan); fetal bovine serum (FBS) (Equitech-Bio, Inc., Kerrville, TX, USA); and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT reagent) (Sigma-Aldrich Co., St. Louis, MO, USA). SN-38 was generously provided by Yakult Honsha Co., Ltd. (Tokyo, Japan). All other chemicals used were of analytical grade.
Tsukamoto M., Yamashita M., Nishi T, & Nakagawa H. (2019). A Human ABC Transporter ABCC4 Gene SNP (rs11568658, 559 G > T, G187W) Reduces ABCC4-Dependent Drug Resistance. Cells, 8(1), 39.
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