The instrumentation used for the investigation of different multiplex injector configurations was a GC-2010 Plus gas chromatograph with a Tracera BID barrier-discharge ionization detector equipped with an external 6-port switching valve from Shimadzu (Kyoto, Japan). The column was a DB-5MS (5% phenyl-95% methyl-polysiloxane) 30 m × 0.25 mm × 0.25 μm from Agilent J&W. A GC-MS-QP2010 Plus instrument (Shimadzu) equipped with a Rt-Q-BOND (100% divinylbenzene, Restek, Bellefonte, PA, USA) PLOT (porous layer-open tubular) column of 30 m × 0.32 mm × 10 μm dimension equipped with a particle trap and a guard column was used for real sample analysis. For the laboratory-made injector, an Arduino Leonardo ETH board (RS Components, Frankfurt am Main, Germany) with a code written in-house for Arduino Software (IDE) v1.8.9 (https://www.arduino.cc/en/main/software , accessed on 30 December 2023) was used for the control of the solenoid valves. The normally open 2-way, normally closed 2-way and 3-way solenoid valves used for the work were purchased from Bürkert Austria GmbH (Vienna, Austria). Tee connectors were from VICI Valco (Schenkon, Switzerland).
Gc ms qp2010 plus instrument
Manufactured by Shimadzu
Sourced in Japan
The GC-MS-QP2010 Plus is a gas chromatography-mass spectrometry (GC-MS) instrument manufactured by Shimadzu. It is designed to perform qualitative and quantitative analysis of complex samples by separating and identifying their chemical components. The instrument combines gas chromatography for sample separation with a quadrupole mass spectrometer for compound detection and identification.
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4 protocols using gc ms qp2010 plus instrument
1
Investigating Multiplex Injector Configurations
2
Quantifying Bromoform in A. taxiformis
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The concentration of CHBr 3 in the oil immersion of A. taxiformis was determined with a procedure adapted from the one reported by Magnusson et al. (2020) (link). Briefly, 1.5 mL of bromoil was mixed with methanol and internal standard (naphthalene, 10 μg/ mL) and analyzed by GC-MS using a Shimadzu GC-MS QP2010-plus instrument (Shimadzu Corp., Kyoto, Japan) equipped with a fused silica capillary column (Supelcowax10, 30 m × 0.25 mm i.d. × 0.20 μm film thickness, Supelco Inc., Bellefonte, PA). Quantification of CHBr 3 was performed with an ionization energy of 70 eV using the selected ion monitoring mode targeting the m/z 173 and m/z 128 quantifier ions for the CHBr 3 and naphthalene, respectively. The CHBr 3 concentration was calculated from its peak area ratio over the internal standard peak area and converted to concentration by reference to standard curves.
3
Lignin Monomer Determination by GC-MS
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EL was degraded by DFRC using previously described experimental conditions (62 (link), 63 ). The DFRC degradation products were acetylated using deuterated acetic anhydride and pyridine [1:1 (v/v), 2 hours], allowing distinction of monomers deriving from originally etherified versus originally free phenolic units (26 (link)) before being analyzed on a Shimadzu GC/MS-QP2010 Plus instrument using previously described chromatographic conditions (62 (link)). Monomer determination was performed by monitoring the m/z as described in (64 (link)): 192, 222, and 252, the base peaks resulting from the loss of ketene (CH2-C=O) for nondeuterated H, G, and S acetates, and 193, 223, and 253 for the deuterated H, G, and S analogs, respectively. The nondeuterated acetate of scopoletin was monitored at m/z 192, and its deuterated analog was monitored at m/z 193. The response of each ion is depicted as an extracted ion chromatogram in Fig. 5 .
4
GC-MS Profiling of Chemical Compounds
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The GC-MS profile was generated using GCMS-QP2010 plus instrument (Shimadzu, Kyoto, Japan) equipped with autoinjector and 5ms capillary column of 30×0.25 mm dimension with 0.25 μm film thickness. Helium served as the carrier gas at 1.15 ml/min. flow rate. Mass spectroscopic analysis was done with 70eV ionization system. The primary temperature was established at 80°C for 2 min. to be gradually elevated at a rate of 10°C per min. up to 280°C for 5 min. The sample injection was according to split mode at 250°C. Two mass spectral databases National Institute of Standards and Technology (NIST14), and Wiley 10th/NIST 2014 mass spectral library (W10N14) adopted in the characterization of the extracted components based on retention time and mass spectra.
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