Prism software v

All data are presented as means ± S.E.M. Comparisons between multiple groups were performed using a two-way ANOVA with a post hoc Fisher’s least significant difference test. Statistical analyses were performed using GraphPad Prism software v9 (GraphPad Software Inc., San Diego, CA, USA).

Data were presented as the mean ± SEM and were analyzed using GraphPad Prism software v5.0 (GraphPad Software, Inc.). Based on Gene Ontology Biological Process (GOBP) definition, Fisher's exact test was applied to determine whether the proportion of differentially expressed genes in a given GOBP gene set was significantly enhanced. RT-qPCR parameters were analyzed using the unpaired t test for repeated measures, with P value less than 0.05 was considered statistically significant.

The early and late apoptotic cell accumulation assay was performed using an Annexin V Detection Kit from e-Biosciences (Thermo Fisher Scientific, Waltham, MA, USA) as per manufacturer instructions. Briefly, the detached cells from the experimental and control groups were incubated with 0.25 µg/mL Annexin V reagent in 1x binding buffer for 15 min followed by 2 washes with a wash buffer. Cells were resuspended in the binding buffer containing 0.5 µg/mL propidium iodide, 10,000 events were acquired immediately using Beckman Coulter Novus Flow Cytometer (Indianapolis, IN, USA) and data were analyzed using Kaluza software (v1.2, Brea, CA, USA). Early- and late-phase apoptotic cells were segregated with a quadriplot graph and the total percentage of apoptotic cells was represented using Graph Pad Prism software V (v5.0, GraphPad Software, San Diego, CA, USA).

Data are presented as mean ± standard error of the mean (SEM). Non-normally distributed variables (failure of the Kolmogorov–Smirnov test) were log2 transformed. Correlations between two variables were tested with parametric or non-parametric tests as specified. Groups’ comparison was performed with one-way ANOVA adjusted for multiple comparisons. The p values less than 0.05 were considered statistically significant. All statistical analysis was performed using GraphPad Prism software v6.0 (GraphPad Inc., San Diego, CA, USA).