S monovette z gel

Manufactured by Sarstedt

Sourced in Germany

The S-Monovette Z-Gel is a laboratory equipment product manufactured by Sarstedt. It is a blood collection tube with a gel separator that helps to isolate serum or plasma from the cellular components of the blood sample.

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Lab products found in correlation

Sars cov 2 igg kit, by EUROIMMUN (3 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) S monovette, by Sarstedt (1 mentions) Tina quant c reactive protein 4, by Roche (1 mentions) Elecsys anti sars cov 2 s, by Roche (1 mentions)

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S-Monovette (283 citations) Monovettes (21 citations) Z-Gel (8 citations) S-Monovette 7.5 mL Z-gel (3 citations) S-Monovette® Z-Gel tubes (3 citations)

10 protocols using s monovette z gel

Biofluid Collection and Cryopreservation

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On the morning of study day 2, fasting plasma samples were collected by drawing blood into 9 mL ethylenediaminetetraacetic acid (EDTA) plasma tubes (S-Monovette, Sarstedt, Nümbrecht, Germany) from an antecubital vein. Blood was centrifuged at 1850 x g at 4°C and aliquoted into small portions. In addition, serum samples (S-Monovette Z-gel, Sarstedt, Nümbrecht, Germany) were collected for standard clinical biochemistry analyses.
Subjects collected 24 h urine, starting the morning prior to study day 2 up to the morning of study day 2. Collection bottles were kept in cool bags with cooling units throughout. Upon delivery of the 24 h urine samples to the study center, the volume was recorded, 2 x 14 mL were centrifuged at 1850 x g at 20°C and aliquoted into small portions.
All samples were initially frozen at -20°C for one day and then cryopreserved at -196°C until analysis as previously found to be an acceptable procedure [25 (link)].
Quality control (QC) samples were prepared by pooling fasting plasma samples and 24 h urine samples, respectively, from KarMeN participants. These QC samples were used for all analytical methods applied.

Rist M.J., Roth A., Frommherz L., Weinert C.H., Krüger R., Merz B., Bunzel D., Mack C., Egert B., Bub A., Görling B., Tzvetkova P., Luy B., Hoffmann I., Kulling S.E, & Watzl B. (2017). Metabolite patterns predicting sex and age in participants of the Karlsruhe Metabolomics and Nutrition (KarMeN) study. PLoS ONE, 12(8), e0183228.

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SARS-CoV-2 IgG Antibody Quantification

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Peripheral blood was collected in S-Monovette Z-Gel (Sarstedt). SARS-CoV-2 IgG titers were analyzed in purified serum using a SARS-CoV-2 IgG kit (Euroimmun, Lübeck, Germany). The test was performed according to the manufacturer’s instructions. Briefly, serum samples were diluted 1:100 and added to plates coated with recombinant SARS-CoV-2 antigen. Bound SARS-Cov-2 S1 protein-reactive IgG was detected by a horseradish peroxidase (HRP)-conjugated anti-human IgG. The absorbance was read on a microplate reader at 450 nm with reference at 620 nm, and evaluated as the ratio of the absorbance of the sample to the absorbance of the internal standard.

Paniskaki K., Anft M., Meister T.L., Marheinecke C., Pfaender S., Skrzypczyk S., Seibert F.S., Thieme C.J., Konik M.J., Dolff S., Anastasiou O., Holzer B., Dittmer U., Queren C., Fricke L., Rohn H., Westhoff T.H., Witzke O., Stervbo U., Roch T, & Babel N. (2022). Immune Response in Moderate to Critical Breakthrough COVID-19 Infection After mRNA Vaccination. Frontiers in Immunology, 13, 816220.

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Neutrophil Phagocytosis of Opsonized S. aureus

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Prior phagocytosis heat inactivated, pHrodo red labeled S. aureus was opsonized in RPMI 1640 medium (Gibco) supplemented with 15% autologous serum for 1 h at 37°C. Autologous serum was freshly isolated from whole blood using S-Monovette Z-Gel (Sarstedt) according to the manufacturer’s instructions. Opsonized bacteria were incubated with neutrophils at MOI of 50 at 37°C in the water bath for 0, 30, and 60 min. Post incubation phagocytosis was stopped by washing cells in ice-cold PBS. Immediately before measurement trypan blue was added to cell suspension and phagocytosis was assessed by flow cytometry.

Serwas N.K., Huemer J., Dieckmann R., Mejstrikova E., Garncarz W., Litzman J., Hoeger B., Zapletal O., Janda A., Bennett K.L., Kain R., Kerjaschky D, & Boztug K. (2018). CEBPE-Mutant Specific Granule Deficiency Correlates With Aberrant Granule Organization and Substantial Proteome Alterations in Neutrophils. Frontiers in Immunology, 9, 588.

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SARS-CoV-2 IgG Antibody Quantification

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Peripheral blood was collected in S-Monovette Z-Gel (Sarstedt). SARS-CoV-2 IgG titers were analyzed in purified serum using a SARS-CoV-2 IgG kit (Euroimmun, Lübeck, Germany). The test was performed according to the manufacturer’s instructions. Briefly, serum samples were diluted 1:100 and added to plates coated with recombinant SARS-CoV-2 antigen. Bound SARS-Cov-2 S1 protein-specific IgG was detected by a horseradish peroxidase (HRP)-conjugated anti-human IgG. The absorbance was read on a microplate reader at 450 nm with reference at 620 nm and evaluated as the ratio the absorbance of the sample to the absorbance of the internal standard.

Anft M., Paniskaki K., Blazquez-Navarro A., Doevelaar A., Seibert F.S., Hölzer B., Skrzypczyk S., Kohut E., Kurek J., Zapka J., Wehler P., Kaliszczyk S., Bajda S., Thieme C.J., Roch T., Konik M.J., Berger M.M., Brenner T., Kölsch U., Meister T.L., Pfaender S., Steinmann E., Tempfer C., Watzl C., Dolff S., Dittmer U., Abou-El-Enein M., Westhoff T.H., Witzke O., Stervbo U, & Babel N. (2020). COVID-19-Induced ARDS Is Associated with Decreased Frequency of Activated Memory/Effector T Cells Expressing CD11a++. Molecular Therapy, 28(12), 2691-2702.

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Urine and Blood Sample Collection

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The day before examinations the participants collected their urine over a period of 24 h. Collection bottles were kept in cool bags with cooling units throughout. The completeness of the 24-h urine collection was verified using the PABA method (para-aminobenzoic acid) through HPLC-UV by the method of Jakobsen et al. [28 (link)]. Upon delivery of the 24-h urine samples in the study center, the volume was recorded. Subsamples were centrifuged at 1850×g at 20 °C and aliquots were stored at − 196 °C until analysis.
Blood samples were collected from an antecubital vein into 9-mL EDTA plasma tubes (S-Monovette, Sarstedt, Nümbrecht, Germany). Blood was centrifuged at 1850×g at 4 °C and aliquoted into small portions. In addition, serum samples (S-Monovette Z-gel, Sarstedt, Nümbrecht, Germany) were collected for standard clinical biochemistry analyses. All samples were initially frozen at − 20 °C for 1 day and then cryopreserved at − 196 °C until analysis. Quality control (QC) samples were prepared by pooling fasting plasma samples and 24-h urine samples, respectively, from KarMeN participants. These QC samples were used for all analytical methods applied.

Armbruster M., Rist M., Seifert S., Frommherz L., Weinert C., Mack C., Roth A., Merz B., Bunzel D., Krüger R., Kulling S., Watzl B, & Bub A. (2018). Metabolite profiles evaluated, according to sex, do not predict resting energy expenditure and lean body mass in healthy non-obese subjects. European Journal of Nutrition, 58(6), 2207-2217.

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Serum Calprotectin Measurement Protocol

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Serum calprotectin was collected in serum-gel vials (S-Monovette Z-Gel, Sarstedt, Nümbrecht, Germany) and measured with EliA™ Calprotectin 2 wells (wells coated with monoclonal mouse antibodies against calprotectin) with a Phadia™ 250 device (both Thermo Fisher Scientific, Freiburg, Germany). For each measurement cycle, a commercial negative and positive control (Thermo Fisher Scientific) was analyzed. Serum samples were centrifuged within 1h after blood collection and stored between 2 and 8°C until measurement. Stable calprotectin values can be measured with this assay within 7 days [22 (link)].
ESR (reference range in the first hour 3–8 mm) and CRP (reference < 0.5 mg/dl) were measured in the central laboratory of the University Hospital of Würzburg. CRP was detected by immunoturbidimetry (Tina-quant C-reactive Protein IV, cobas, Roche, Mannheim, Germany).

Gernert M., Schmalzing M., Tony H.P., Strunz P.P., Schwaneck E.C, & Fröhlich M. (2022). Calprotectin (S100A8/S100A9) detects inflammatory activity in rheumatoid arthritis patients receiving tocilizumab therapy. Arthritis Research & Therapy, 24, 200.

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SARS-CoV-2 IgG Antibody Titer Assay

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Peripheral blood was collected in S-Monovette Z-Gel (Sarstedt). SARS-CoV-2 IgG titers were analyzed in purified serum using a SARS-CoV-2 IgG kit (Euroimmun, Lübeck, Germany). The test was performed according to the manufacturer's instructions. Briefly, serum samples were diluted 1:100 and added to plates coated with recombinant SARS-CoV-2 antigen. Bound SARS-CoV-2 S1 protein-reactive IgG was detected by horseradish peroxidase-conjugated anti-human IgG. The absorbance was assessed on a microplate reader at 450 nm with a reference at 620 nm and evaluated as the ratio of the absorbance of the sample to the absorbance of the internal standard.

Paniskaki K., Anft M., Thieme C.J., Skrzypczyk S., Konik M.J., Dolff S., Westhoff T.H., Nienen M., Stittrich A., Stervbo U., Witzke O., Heine G., Roch T, & Babel N. (2022). Severe Acute Respiratory Syndrome Coronavirus 2 Cross-Reactive B and T Cell Responses in Kidney Transplant Patients. Transplantation Proceedings, 54(6), 1455-1464.

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Serum Biomarkers in Allogeneic Stem Cell Transplantation

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Pre-transplant serum samples were collected in gel tubes (S-Monovette® Z-Gel, SARSTEDT AG & Co. KG, Nuembrecht, Germany) before alloSCT and cryopreserved at −80 °C. In the training cohort, post-transplant serum samples were also available. Serum levels of total IL-18, IL-18-binding protein A (IL-18BPa) and free IL-18 as well as C-X-C motif ligand 9 (CXCL9)/monokine induced by gamma-interferon (MIG) and soluble suppression of tumorigenecity 2 (ST2) levels were assessed prior to the start of pre-transplant conditioning in both cohorts. IL-18, IL-18BPa, and free IL-18 were determined on days 0, +3, +4, +7, +8, +12, +21, and +28 after alloSCT in the training cohort. For a detailed description of the methodology and the SNP analyses please refer to the Supplement (section on Methods).

Radujkovic A., Kordelas L., Dai H., Schult D., Majer-Lauterbach J., Beelen D., Müller-Tidow C., Dreger P, & Luft T. (2019). Interleukin-18 and outcome after allogeneic stem cell transplantation: A retrospective cohort study. EBioMedicine, 49, 202-212.

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Serum Biomarkers in SARS-CoV-2 Infection

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Serum samples were collected in gel tubes (S-Monovette^® Z-Gel, SARSTEDT AG & Co. KG, Nuembrecht, Germany) at the time SARS-CoV-2 testing and cryopreserved at −80°C. Serum levels of soluble thrombomodulin (sTM, sCD141), suppressor of tumorigenicity 2 (ST2), Angiopoietin-2 (Ang2), chemokine-X-C-ligand 8 (CXCL8, interleukin 8), CXCL9 (monokine induced by gamma interferon, MIG), interleukin-18 (IL18), interleukin-18 binding protein A (IL18BPa), and interferon-alpha (IFNα) were assessed by ELISA using commercial kits (DuoSet, R&D Systems, Wiesbaden, Germany) according to the manufacturer’s instructions as reported previously (19 (link), 20 (link)).

Luft T., Wendtner C.M., Kosely F., Radujkovic A., Benner A., Korell F., Kihm L., Bauer M.F., Dreger P, & Merle U. (2021). EASIX for Prediction of Outcome in Hospitalized SARS-CoV-2 Infected Patients. Frontiers in Immunology, 12, 634416.

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Neutralizing Antibody Assay for SARS-CoV-2

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Serum was generated from blood collected into S-Monovette Z-Gel (Sarstedt). The SARS-CoV-2 wild-type neutralisation assay was performed as previously described.23 (link) Briefly, pseudoviruses were incubated with twofold serial dilutions from 1:20 to 1:2560 of immune sera in 96-well plates prior to infection of Vero E6 cells (1×10⁴ cells/well). At 18 hours postinfection, firefly luciferase activity was determined as a proxy for infection and the reciprocal antibody dilution causing 50% inhibition of the luciferase reporter was calculated (ND50). Detection range was defined to be between 1:20 and 1:2 560, meaning that patients with a ND50 less than 1:20 were classified as having no neutralising antibodies.
The Elecsys Anti-SARS-CoV-2 S (Roche Diagnostics, Switzerland) immunoassay was used for measurement of IgG to SARS-CoV-2 spike-protein. This assay measures the presence and amount of serum antibodies to the spike (S) antigen of SARS-CoV-2 and reports these in the units (U)/mL, which is equivalent to BAU/mL. The assay has a linear detection range up to 250 U/mL. By prediluting the samples 1:50, a concentration range up to 25 000 U/mL can be reached. This strategy was applied to samples obtained at ‘2nd dose+4 weeks’ to allow evaluation of a wider range after vaccination. Seroconversion was defined as a response equal to or greater than 0.8 U/mL.

Andreica I., Blazquez-Navarro A., Sokolar J., Anft M., Kiltz U., Pfaender S., Vidal Blanco E., Westhoff T., Babel N., Stervbo U, & Baraliakos X. (2022). Different humoral but similar cellular responses of patients with autoimmune inflammatory rheumatic diseases under disease-modifying antirheumatic drugs after COVID-19 vaccination. RMD Open, 8(2), e002293.

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