For mutant cyt c the expression plasmids of horse cyt c (pHCyc) [50 (link)] was subjected to one round of mutagenesis with QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara United States), which introduced Lys72Asn substitution into the horse cyt c gene. Mutant pHCyc plasmids was introduced into E. coli JM 109. Protein expression and purification of the recombinant protein were then conducted as previously described [51 (link)].
Bovine heart cardiolipin
Bovine heart cardiolipin is a lipid compound found in the inner membrane of mitochondria. It is extracted from the heart tissue of bovine (cattle) origin. Cardiolipin plays a crucial role in the proper functioning of mitochondria and is essential for cellular energy production.
Lab products found in correlation
3 protocols using bovine heart cardiolipin
Purification and Characterization of Mutant Cytochrome c
For mutant cyt c the expression plasmids of horse cyt c (pHCyc) [50 (link)] was subjected to one round of mutagenesis with QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara United States), which introduced Lys72Asn substitution into the horse cyt c gene. Mutant pHCyc plasmids was introduced into E. coli JM 109. Protein expression and purification of the recombinant protein were then conducted as previously described [51 (link)].
Enzymatic Synthesis of 20(OH)D3 and 20,23(OH)2D3
Lipid Peroxidation and Membrane Biophysics
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!