Anti drp1

Anti-Tim23 (1/1,000), anti-Tom20 (for western blots, 1/1,000, for immunofluorescence, 1/200), anti-Bcl2-L-13 (1/1,000) (Proteintech), anti-cytochrome c (1/1,000) (BD biosciences), anti-Bcl2-L-13 (1/500), anti-GAPDH (1/1,000), anti-Fis1 (1/1,000), anti-α-tubulin (1/1,000) (Abcam), anti-LC3B (for western blots, 1/1,000, for immunofluorescence, 1/100), anti-HA (for western blots, 1/1,000, for immunofluorescence, 1/200), anti-Drp1Ser637 (1/500), anti-Drp1Ser616 (1/1,000), anti-Mfn2 (1/1,000), anti-cleaved caspase 3 (1/1,000) (Cell Signaling Technology), anti-ATP synthase subunit beta (for western blots, 1/1,000, for immunofluorescence, 1/200) (Life Technologies), anti-Drp1 (for western blots, 1/1,000, for immunofluorescence, 1/100), anti-Opa1 (1/1,000) (BD Transduction Laboratories), anti-FLAG (for immunofluorescence, 1/200) (Sigma), anti-Mfn1 (1/1,000) (Abnova), anti-ubiquitin (for immunofluorescence, 1/200) (Enzo Life Science), anti-Phospho-Serine/Threonine (Upstate), anti-mCherry (1/500) (Clontech) and anti-RFP23 (link) (1/1,000) antibodies were used in this study.

Fluo4-AM, calcein-AM, MitoTracker Orange CMTMRos and Alexa Fluor^®488 anti-mouse were from Molecular Probes (Carlsbad, CA, USA). Neurobasal medium, B27 supplement, and horse serum were from Gibco (Carlsbad, CA, USA). Dulbeco’s modified essential medium (DMEM) and lipofectamine 2000 were from Invitrogen (Carlsbad, CA, USA). Ryanodine was from Alexis (Lausen, Switzerland). The mito-pericam plasmid was a kind gift from Dr. Eisner. Protease inhibitors leupeptin and pepstatin A were from Calbiochem (La Jolla, CA, USA), and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) was from Sigma Chemical (St Louis, MO, USA). Bicinchoninic acid assay kit, horseradish peroxidase (HRP)-conjugated secondary antibodies and m-Hsp-70 antibody were from Pierce Biotecnology (Rockford, IL, USA), 4-cloro-methyl-cresol was from Merck (Darmstadt, Germany) and polyvinylidene difluoride (PDVF) membranes from Millipore (Bedford, MA, USA). The antibody anti-Cox IV was from Cell Signaling (Denvers, MA, USA), anti-Drp-1 was from Thermo Scientific (Salt Lake City, UT, USA), anti m-Hsp-70 was from Pierce Biotecnology (Rockford, IL, USA).