Female mice in estrus were identified using vaginal lavage, followed by cytological staining (Giemsa solution, Polysciences) and visual assessment. Male mice were habituated to the experimental arena (40 × 20 cm) for 3 min. Next, an unfamiliar female mouse in estrus was added to the arena, and the mice were allowed to interact freely for 3 min. Ultrasonic vocalizations (USVs) between 40 and 250 Hz produced by the male mice were recoded using a preamplifier (UltraSoundGate 416 H, Avisoft Bioacoustics) connected to a microphone (UltraSoundGate CM16, Avisoft Bioacoustics). The total number of USVs and their duration was analyzed using SASLabPro (Avisoft Bioacoustics).
Giemsa solution
Manufactured by Polysciences
Giemsa solution is a laboratory reagent used for the staining of blood smears and other cell samples for microscopic examination. It is a polychromatic stain that differentially colors various cellular components, allowing for the identification and visualization of different cell types.
Automatically generated - may contain errors
3 protocols using giemsa solution
1
Courtship Ultrasonic Vocalizations in Mice
2
Social Interaction in Mice Across Development
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At P21, test pups were habituated to the arena for 3 min. Subsequently, WT females in estrus, unfamiliar to the pups, were added to the cage, and both mice were allowed to interact with each other for another 3 min in a well lit room. The interaction between the pups and the females was recorded using a computer-linked video camera (The Imaging Source) located above the arena. Videos were manually scored, and interaction was recorded when both mice were within 2 cm of each other, not including tail–tail interactions.
At P60, only female mice were tested for social interaction. In this case the unknown WT females were not required to be in estrus.
To determine which females were in estrus, vaginal smears were stained with Giemsa solution (Polysciences; Caligioni, 2009 ) before the experiment.
At P60, only female mice were tested for social interaction. In this case the unknown WT females were not required to be in estrus.
To determine which females were in estrus, vaginal smears were stained with Giemsa solution (Polysciences; Caligioni, 2009 ) before the experiment.
3
Social Interaction in Mice: Developmental Dynamics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At P21, test pups were habituated to the arena for 3 min. Subsequently, WT females in estrous, unfamiliar to the pups, were added to the cage and both mice were allowed to interact with each other for another 3 min in a well-lit room. The interaction between the pups and the females was recorded using a computer-linked video camera (The Imaging Source) located above the arena. Videos were manually scored, and interaction recorded when both mice were within 2 cm of each other, not including tail-tail interactions.
At P60, only female mice were tested for social interaction. In this case the unknown WT females were not required to be in oestrus.
To determine which females were in oestrus, vaginal smears were stained with Giemsa solution (Polysciences inc.) (Caligioni 2009) prior to the experiment.
At P60, only female mice were tested for social interaction. In this case the unknown WT females were not required to be in oestrus.
To determine which females were in oestrus, vaginal smears were stained with Giemsa solution (Polysciences inc.) (Caligioni 2009) prior to the experiment.
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