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Autopure ls nucleic acid purification system

Manufactured by Qiagen
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The Autopure LS nucleic acid purification system is a laboratory instrument designed for the automated extraction and purification of nucleic acids, including DNA and RNA, from a variety of sample types. The core function of the Autopure LS is to provide a streamlined and reliable process for isolating high-quality nucleic acids, which are essential for a wide range of downstream applications in research, diagnostics, and molecular biology.

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Lab products found in correlation

Oragene dna collection kit, by DNA Genotek (3 mentions) Taqman snp genotyping assay, by Thermo Fisher Scientific (1 mentions) Iplex gold, by Labcorp (1 mentions) Massarray system, by Labcorp (1 mentions)

Close spelling variants of this product

Autopure LS (70 citations) Autopure LS system (20 citations) Autopure (20 citations) Autopure System (6 citations)

4 protocols using autopure ls nucleic acid purification system

1

Genetic Polymorphism Analysis from Blood

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A single venous blood sample (9 mL) was collected from each patient either pre- or post-therapy. Genomic DNA was isolated from leukocytes using the Autopure LS nucleic acid purification system (QIAgen, Hilden, Germany) at Genetic Repositories Australia, Sydney. DNA isolates were then amplified and used in separate assays to genotype each sample for the BDNF-Val66Met polymorphism and APOE-ε4 allele, as described below.
Shiner C.T., Pierce K.D., Thompson-Butel A.G., Trinh T., Schofield P.R, & McNulty P.A. (2016). BDNF Genotype Interacts with Motor Function to Influence Rehabilitation Responsiveness Poststroke. Frontiers in Neurology, 7, 69.
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2

Genotyping of CETP and APOE Variants

Cited 1 time
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Genomic DNA from saliva samples was obtained using the Oragene DNA collection kit (DNA Genotek, Ottawa, Canada). Samples were shipped to Genetic Repositories Australia at Neuroscience Research Australia for processing using the Autopure LS nucleic acid purification system (QIAgen, Hilden, Germany).
The CETP I405V polymorphism (rs5882) and APOE SNPs at positions 112 (rs429358) and 158 (rs7412) of the peptide sequence (Weisgraber et al. 1981 (link)) were genotyped using the Sequenom MassArray system. APOE isoform genotypes were assigned as previously described (Salminen et al. 2013 (link)). The allelic frequencies of all SNPs were within Hardy-Weinberg equilibrium (rs5882, χ2 = 1.32, df.= 1, p = 0.25; rs429358, χ2 = 3.43, df = 1, p = 0.06; rs7412, χ2 = 2.77, df = 1, p = 0.1).
Salminen L.E., Schofield P.R., Pierce K.D., Luo X., Zhao Y., Laidlaw D.H., Cabeen R.P., Conturo T.E., Lane E.M., Heaps J.M., Bolzenius J.D., Baker L.M., Cooley S.A., Scott S., Cagle L.M, & Paul R.H. (2015). Genetic markers of cholesterol transport and gray matter diffusion: A preliminary study of the CETP I405V polymorphism. Journal of neural transmission (Vienna, Austria : 1996), 122(11), 1581-1592.
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3

Genetic Variation in Oxidative Stress

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Genetic isolation and processing was completed at Genetics Repositories Australia. Genomic DNA was extracted from saliva samples using the Oragene DNA collection kit (DNA Genotek, Ottawa, Canada) and the Autopure LS nucleic acid purification system (QIAgen). Genotypes for SOD2 and CAT were ascertained using predesigned Taqman SNP Genotyping Assays (Applied Biosystems) according to the manufacturer's protocol: SOD2 rs4880 (Assay ID C_8709053_10) CAT-262 rs1001179 (Assay ID C_11468118_10).
The following allele frequencies were observed: SOD2: CC n = 26, CT n =43, TT n=27; CAT: CC n=61, CT n=32, TT n= 3. Genotype frequencies for both SNPs did not deviate from Hardy-Weinberg equilibrium (rs4880, χ2 = 1.04, df = 1, p = 0.308; rs1001179, χ2 = 0.24, df = 1, p = 0.064). Given the cell sizes for CAT, a dominant grouping system was used to compare individuals with the CC genotype against individuals with the CT/TT genotypes. While these genotypes are arranged according to a recessive grouping system, this model is functionally dominant as the C allele is far more common than the minor T allele and thus is inferred to have the dominant effect. For SOD2, preliminary analyses indicated that the recessive model was the most robust to brain outcomes in this study. Thus, individuals with the CC genotype of SOD2 were compared against individuals with the CT/TT genotypes.
Salminen L.E., Schofield P.R., Pierce K.D., Bruce S.E., Griffin M.G., Tate D.F., Cabeen R.P., Laidlaw D.H., Conturo T.E., Bolzenius J.D, & Paul R.H. (2017). Vulnerability of white matter tracts and cognition to the SOD2 polymorphism: A preliminary study of antioxidant defense genes in brain aging. Behavioural brain research, 329, 111-119.
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4

Saliva DNA Extraction and Genotyping

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Saliva samples were collected during the initial neuropsychological evaluation using the Oragene DNA collection kit (DNA Genotek, Ottawa, Canada) and shipped to Genetic Repositories Australia at Neuroscience Research Australia for processing. Genomic DNA was extracted from saliva samples using the Autopure LS nucleic acid purification system (QIAgen, Hilden, Germany).
The AGT genotypes at the rs699 polymorphism were determined using iPLEXGold™ primer extension followed by mass spectrometry analysis on the Sequenom Mass ARRAY system (Sequenom, San Diego, CA) by the Australian Genome Research Facility (http://www.agrf.org.au/).
Genotyping results of the larger sample from which participants were extracted revealed an allele distribution that did not differ from that expected under Hardy-Weinberg equilibrium [χ2(1, N = 103) = 1.17, p = 0.279]. The allele frequency for both Met and Thr alleles was 0.50. Observed genotype frequencies were MetMet=0.28, MetThr=0.45, ThrThr= 0.27. Homozygous subsets (n=27 for each homozygous genotype) were selected from within this group for the present study.
Salminen L.E., Schofield P.R., Pierce K.D., Zhao Y., Luo X., Wang Y., Laidlaw D.H., Cabeen R.P., Conturo T.E., Tate D.F., Akbudak E., Lane E.M., Heaps J.M., Bolzenius J.D., Baker L.M., Cagle L.M, & Paul R.H. (2015). Neuromarkers of the common angiotensinogen polymorphism in healthy older adults: A comprehensive assessment of white matter integrity and cognition. Behavioural brain research, 296, 85-93.
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