Diphenyleneiodonium dpi

Doxycycline (Dox) was purchased from AppliChem (Darmstadt, Germany), recombinant human IL-1Ra from Genzyme (Cambridge, MA, USA), recombinant human IL-1β from PeproTech (London, UK), Ac-YVAD-CMK from Bachem (Torrance, CA, USA), bafilomycin A1 from Fermenteck (Jerusalem, Israel), and CA-074Me from Merck (Darmstadt, Germany). Diphenyleneiodonium (DPI) was purchased from Enzo (Plymouth Meeting, PA, USA). LPS from Escherichia coli K235 was purchased from Sigma-Aldrich (St. Lois, MO, USA). ATP was purchased from YAMASA CORPORATION (Chiba, Japan). Cycloheximide (CHX) and MSU crystals were purchased from Wako Pure Chemical Industries (Osaka, Japan). Aluminum adjuvant (Imject Alum) was purchased from Pierce (Rockford, IL, USA). Ficoll-Paque PLUS was purchased from GE Healthcare Japan (Tokyo, Japan).

TPA and HRP-conjugated goat anti-rabbit (A6154) and anti-mouse (A4416) IgG were purchased from Sigma-Aldrich Co. (St. Louis, MO). A PKC inhibitor (GF109203X) and actinomycin D (ActD) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Cyclopentylidene-(4-(4′-chlorophenyl)thiazol-2-yl)hydrazone (CPTH2) was purchased from Calbiochem (San Diego, CA). 5-(and-6)-Carboxy-2′,7′-dichlorodihydrofluorescein diacetate (carboxy-H₂DCFHDA) and dihydroethidium (DHE) were purchased from Molecular Probes (Eugene, OR). Diphenyleneiodonium (DPI) and garcinol (Gar) were purchased from Enzo Life Sciences Inc. (Farmingdale, NY). Trichostatin A (TSA) was purchased from Cayman Chemical (Ann Arbor, MI). An anti-phospho-PKC (pan) (βII Ser660) rabbit polyclonal antibody (#9371) and normal rabbit IgG (#2729) were purchased from Cell Signaling Technology (Danvers, MA). Anti-actin mouse monoclonal antibody (MAB1501) and anti-acetyl-histone H3 (#06-599) and H4 (#06-598) rabbit polyclonal antibodies were purchased from Millipore Co. (Billerica, MA).