A male moth was restrained in a plastic pipette tip with only the head protruding from the aperture, and a tungsten wire serving as a reference electrode was inserted into the abdomen. Single sensillum recordings (SSRs) were performed under a light microscope (Nikon FN-S2N) with 750x magnification, using tungsten electrodes (Clark Instruments Ltd). The recording electrode was attached to an AC/DC 10× gain probe (INR-02; Syntech), and its tip was inserted at the base of a pheromone-sensitive long trichoid sensillum using a micromanipulator (Märzhauser PM-10) until extracellular electrical contact with olfactory sensory neurons (OSNs) was established. The signal was amplified, digitized (IDAC-4 USB; Syntech) and visualized with AutoSpike 3.7 software (Syntech). A stream of charcoal filtered humidified air was continuously flushed over the antenna (1 L.min−1), through a glass tube (1.0 mm i.d.), which terminated 2.0 cm from the antenna. During stimulation, a 0.5-s air pulse (1.0 L min−1) controlled by a stimulus controller (CS-55; Syntech) was passed through the stimulus pipette, which was inserted into a hole in the glass tube. Compounds were tested with an inter-stimulus interval of at least 1 min. The response of OSNs was expressed as the number of spikes during the stimulation period after stimulus onset minus the number of spikes before stimulus onset.
Idac 4 usb
Manufactured by Syntech
Sourced in Germany
The IDAC-4-USB is a high-performance data acquisition device that provides four independent analog input channels. It is designed for data logging and measurement applications, offering a USB interface for easy connectivity to a computer. The device features a 16-bit analog-to-digital converter and can sample data at rates up to 100 kHz per channel.
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Lab products found in correlation
7 protocols using idac 4 usb
1
Single Sensillum Recording of Moth Pheromone Responses
2
Electroantennogram Assay for Moth Sex Pheromones
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The EAG assays were performed on both sexes of Helicoverpa armigera and Spodoptera frugiperda respectively according to previous reports (Guo et al., 2021 (link)). The antennae of 3-day-old virgin male and female moths were cut at the base of the flagellum. About 1 μg/μL stock solutions of individual compound was prepared with n-hexane. In each test, 10 μg of each compound was used for stimulation. Odor stimulation was controlled by a puff of purified air (0.2 s at 10 mL/s airflow) from a stimulus controller (CS-55, Syntech, Kirchzarten, Germany). EAG signals were recorded and monitored with an Intelligent Data Acquisition Controller (IDAC-4-USB, Syntech), then analyzed using Syntech EAG-software. For each compound, 4 or 5 antennae from different individual moths were tested. The EAG response values of each compound were calculated by subtracting that of the same antennae corresponding to a solvent blank of n-hexane.
3
Electroantennogram Recording of P. xylostella
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The antennae of P. xylostella adults (female and male moths) were cut off from the base, and tip segments were removed at the end. The glass electrode (KCl, 0.1 mol/L) was used to connect the antennae to the electroantennogram, which consists of an air stimulus controller (CS-55, Syntech, Hilversum, Netherlands), 10 × AC/DC Headstage Preamplifier (Syntech, Hilversum, Netherlands) and Intelligent Data Acquisition Controller (IDAC-4-USB, Syntech, Hilversum, Netherlands). The Syntech EAG software 2.0 was used to recorded (Syntech, Hilversum, Netherlands). Benzyl alcohol, salicylaldehyde, and phenylacetaldehyde were diluted in hexane at concentrations of 10, 100 and 1,000 ng/μL. Take 10 μL test solution on the filter paper strip (1 × 5 cm) and placed into a Pasteur pipette. The antenna was exposed to a constant charcoal-filtered humid air flow (500 ml/min) through a metal tube for a stimulation time of 0.2 s. The female and male moths were repeated 10 times each. EAG responses for each compound were calculated by EAG response to the test compound EAG response to the blank. Data analysis used mean ± standard error of the mean (SEM) and independent t-test.
4
Electrophysiological Assay of Moth Pheromone Response
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The electrophysiological recordings of whole male and female antennae in response to four sex pheromone components and pheromone analogs were performed according to the standard technique (Cao et al., 2016 (link)). The components used in the EAG assay were dissolved in paraffin oil and diluted to 10 μg/μL. A piece of filter paper (0.5 × 5 cm) loaded with 10 μL pheromones was used as a stimulus, and paraffin oil was used as a control. 3-day-old moths were tested and signals from antennae were amplified with a 10 × AC/DC headstage preamplifier (Syntech, Kirchzarten, Germany) and further acquired with an Intelligent Data Acquisition Controller (IDAC-4-USB; Syntech, Kirchzarten, Germany). Signals were recorded with Syntech EAG-software (EAGPro 2.0). After subtracting the responses of the control, data were analyzed using the Student’s t-test (El-Sayed et al., 2009 (link)).
5
Electrophysiological Recording of Antennal Activity in Drosophila
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Female wild type flies were immobilized on ice and placed in a pipet tip exposing the fly’s head through the tip opening. Two glass capillaries equipped with a silver wire in a silver-chloride solution were used to record antennal activity. One electrode inserted into the eye served as reference electrode. The recording electrode was connected to an EAG-probe (Syntech, Germany) and 10x pre-amplified. The electrode tip was attached onto the 3rd antennal segment. The recorded signal was further amplified by a high impedance DC-Amplifier and digitally converted with a sampling rate of 10 kHz (IDAC-4 USB, Syntech) using a dedicated software (GC-EAD, Syntech, Germany).
6
Electrophysiological Odorant Response in Moths
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The antennae of 2- to 3-day-old virgin male and female moths were cut at the base of the flagellum. After removing the tip, one antenna was inserted between two glass electrodes filled with 0.1 M KCl solution. In each test, a 10-μl solution of each odorant was added onto a piece of filter paper (0.5 cm × 0.5 cm) and then inserted into a Pasteur pipette. About 100 ng/μl stock solutions of individual odorants and different doses of the mixture IAC were prepared with paraffin oil. A continuous airflow of 30 ml/s was produced by a stimulus controller (CS-55, Syntech, Kirchzarten, Germany). Odor stimulation was controlled by a puff of purified air (0.2 s at 10 ml/s airflow) from the CS-55. EAG signals were amplified with a 10× AC/DC headstage preamplifier (Syntech) and further acquired with an Intelligent Data Acquisition Controller (IDAC-4-USB, Syntech) (Cao et al. 2016 (link)). The signals were recorded, monitored, and analyzed using Syntech EAG-software (Syntech, Germany). The EAG response values for each compound were calculated by subtracting the value of the same antennae corresponding to a solvent blank of paraffin oil.
7
Electrophysiological Responses to Pheromones
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The electrophysiological recordings of whole male and female antennae in response to conspecific sex pheromone components and other species pheromone compounds were conducted according to the standard technique (Cao et al. 2016 ).
The components used in the EAG assay were dissolved in paraffin oil and diluted to 10 μg/μL. A piece of filter paper (0.5 × 5 cm) loaded with 10 μL pheromones was used as a stimulus, and paraffin oil was used as a control. Moths captured from the field were tested, and signals from antennae were amplified with a 10 × AC/DC headstage preamplifier (Syntech, Kirchzarten, Germany) and further acquired with an Intelligent Data Acquisition Controller (IDAC-4-USB; Syntech, Kirchzarten, Germany). Signals were recorded using SyntechEAG-software (EAGPro 2.0).
The components used in the EAG assay were dissolved in paraffin oil and diluted to 10 μg/μL. A piece of filter paper (0.5 × 5 cm) loaded with 10 μL pheromones was used as a stimulus, and paraffin oil was used as a control. Moths captured from the field were tested, and signals from antennae were amplified with a 10 × AC/DC headstage preamplifier (Syntech, Kirchzarten, Germany) and further acquired with an Intelligent Data Acquisition Controller (IDAC-4-USB; Syntech, Kirchzarten, Germany). Signals were recorded using SyntechEAG-software (EAGPro 2.0).
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