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Anti mouse cd40 clone 1c10

Manufactured by Southern Biotech
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The anti-mouse CD40 (clone 1C10) is a laboratory reagent used in flow cytometry and other immunological applications. It binds specifically to the CD40 receptor expressed on mouse cells.

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Lab products found in correlation

Polyinosinic polycytidylic acid poly 1 c, by Merck Group (3 mentions) H 2kb, by BioLegend (1 mentions)

Close spelling variants of this product

Anti–mouse CD40

3 protocols using anti mouse cd40 clone 1c10

1

Evaluating VHH-E7 Immunotherapy for HPV Tumors

Cited 1 time
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To study HPV-induced tumors, age-matched, 6-week-old female C57BL/6J mice were inoculated with 3×105 C3.43 cells. When tumors were palpable (~14 days later; see average tumor growth in Supplemental Figure 5), mice were treated intraperitoneally with VHHCD11b-E7 plus adjuvant or adjuvant only. The adjuvant was 50 μg polyinosinic-polycytidylic acid (Poly(I:C) (Sigma Aldrich) and 50 μg anti-mouse CD40 (clone 1C10; Southern Biotech) as used previously.22 (link) For mice inoculated with both C3.43 and B16 cells, C3.43 cells were injected 6 days prior to inoculation with 1×105 B16 melanoma cells as B16 cells grow faster in vivo. Mice were treated with VHHCD11b-E7 plus adjuvant or adjuvant only at ~14 days post C3.43 challenge (8 days post B16 challenge). Seven days later, mice were given the 64Cu-labeled HPV E7 synTac by retroorbital injection and imaged by PET the following day (8 days post treatment). Following imaging, mice were euthanized and splenocytes and tumor cells were harvested, enumerated, and used for ELISpot analyses where indicated.
Woodham A., Zeigler S., Zeyang L., Kolifrath S., Cheloha R., Rashidian M., Chaparro R., Seidel R., Garforth S., Dearling J., Mesyngier M., Duddempudi P., Packard A., Almo S, & Ploegh H. (2020). In vivo detection of antigen-specific CD8 T cells by immuno-positron emission tomography. Nature methods, 17(10), 1025-1032.
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2

Flow Cytometry Immunophenotyping of Activated Murine Cells

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Rat anti-CD11b-PE (clone M1/70) was purchased from eBioscience (San Diego, CA). Anti-mouse H-2Db, H-2Kb, and CD80 were purchased from BioLegend (San Diego, CA). Anti-mouse CD40 (clone 1C10) was purchased from Southern Biotech (Birmingham, AL). polyinosinic-polycytidylic acid (Poly(I:C)) and lipopolysaccharide (LPS) from Escherichia coli (E.coli) were purchased from Sigma Aldrich (St. Louis, MO).
Woodham A.W., Cheloha R.W., Ling J., Rashidian M., Kolifrath S.C., Mesyngier M., Duarte J.N., Bader J.M., Skeate J.G., Da Silva D.M., Kast W.M, & Ploegh H.L. (2018). Nanobody-antigen conjugates elicit HPV-specific antitumor immune responses. Cancer immunology research, 6(7), 870-880.
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3

Evaluating VHH-E7 Immunotherapy for HPV Tumors

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
To study HPV-induced tumors, age-matched, 6-week-old female C57BL/6J mice were inoculated with 3×105 C3.43 cells. When tumors were palpable (~14 days later; see average tumor growth in Supplemental Figure 5), mice were treated intraperitoneally with VHHCD11b-E7 plus adjuvant or adjuvant only. The adjuvant was 50 μg polyinosinic-polycytidylic acid (Poly(I:C) (Sigma Aldrich) and 50 μg anti-mouse CD40 (clone 1C10; Southern Biotech) as used previously.22 (link) For mice inoculated with both C3.43 and B16 cells, C3.43 cells were injected 6 days prior to inoculation with 1×105 B16 melanoma cells as B16 cells grow faster in vivo. Mice were treated with VHHCD11b-E7 plus adjuvant or adjuvant only at ~14 days post C3.43 challenge (8 days post B16 challenge). Seven days later, mice were given the 64Cu-labeled HPV E7 synTac by retroorbital injection and imaged by PET the following day (8 days post treatment). Following imaging, mice were euthanized and splenocytes and tumor cells were harvested, enumerated, and used for ELISpot analyses where indicated.
Woodham A., Zeigler S., Zeyang L., Kolifrath S., Cheloha R., Rashidian M., Chaparro R., Seidel R., Garforth S., Dearling J., Mesyngier M., Duddempudi P., Packard A., Almo S, & Ploegh H. (2020). In vivo detection of antigen-specific CD8 T cells by immuno-positron emission tomography. Nature methods, 17(10), 1025-1032.
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