Veleta 4 megapixel side mount ccd camera

For ultrastructural examination, representative or interesting areas were selected based on light-microscopical assessment. Ultrathin (60–90 nm) sections were cut with a diamond knife (Ultra 45°, DiATOME) on an Ultrotome III (LKB) and collected upon Pioloform-coated, single-slot copper grids. The sections were contrast-stained with 7% uranyl acetate in 70% methanol and Reynolds’ lead citrate solution and examined in a FEI Tecnai 12 transmission electron microscope (operating at 80 kV) equipped with a Veleta 4-megapixel side-mount CCD camera (Olympus Soft Imaging Solutions GmbH).
Digital images were acquired with TIA software (TEM Imaging & Analysis, version 4.7 SP3; FEI). Alternatively, sets of serial digital images with 10% overlap (at an original magnification of ×30,000) were acquired with the automated EM data acquisition software package SerialEM^{133 (link)} (https://bio3d.colorado.edu/SerialEM). Final alignment (stitching) of the sets of overlapping images and montage blending were done using the reconstruction and modeling software package IMOD^{123 (link)} (https://bio3d.colorado.edu/imod) operating under the Unix toolkit Cygwin. Montages or single images were finally saved as TIFF and imported into Fiji ImageJ for further image processing, examination and selection of interesting areas. Images were assembled into figures using Adobe Photoshop.