Inverted microscope

Cell migration was evaluated using a scratch (wound) healing assay as in our previous study [20 (link)]. In brief, HUVEC monolayers were wounded with a 200 μL pipette tip. Cells were then maintained for 8 h in basal medium containing PBS or Ang-1 (300 ng/mL). Wounded areas were visualized with an Olympus inverted microscope and quantified using Image-Pro Plus™ 8.0 software (Media Cybernetics, Bethesda, MD, USA). Values are reported as percent wound healing, which were calculated using the following formula:
where t8 is the time (8 h) over which cells were maintained in the medium and t0 is the time immediately after wounding.

EC migration was measured using a scratch (wound) healing assay as previously described (Abdel-Malak et al., 2008 (link)). GapmeR-transfected HUVECs were grown as monolayers and then wounded with a 200-μl pipette tip. Cells were maintained in basic medium containing PBS or Ang-1 (300ng/ml) for 8h. Wounded areas were imaged using an Olympus inverted microscope and quantified using Image-Pro Plus™ software (Media Cybernetics, Bethesda, MD). Values are reported as % wound healing and calculated according to the following formula:
where t8 is the time (8h) over which cells were maintained in media and t0 is the time immediately following wounding.

EC migration was measured using a scratch (wound) healing assay as previously described (Abdel-Malak et al., 2008b (link)). siRNA-transfected HUVECs were grown as monolayers then wounded with a 200 μl pipette tip. Cells were maintained in basic medium containing PBS or Ang-1 (300 ng/ml) for 8 h. Wounded areas were imaged using an Olympus inverted microscope and quantified using Image-Pro Plus^TM software (Media Cybernetics, Bethesda, MD). Values are reported as% wound healing, calculated according to the following formula:
% wound healing = [1—(wound area at t8/wound area at t₀)] × 100
where t₈ is the time (8 h) over which cells were maintained in media and t₀ is the time immediately following wounding.