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Ab54793

Manufactured by Abcam
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Ab54793 is a laboratory reagent product offered by Abcam. It is a specific antibody designed for use in research applications. The core function of this product is to facilitate the detection and analysis of target molecules in laboratory experiments.

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Lab products found in correlation

Automated staining platform, by Roche (2 mentions) Envision polymer, by Agilent Technologies (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Ab50692, by Abcam (1 mentions) Ab223868, by Abcam (1 mentions) Ab208775, by Abcam (1 mentions)

4 protocols using ab54793

1

Immunohistochemical Analysis of ATR

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Immunohistochemistry for ATR was done on formalin-fixed, paraffin-embedded tissues using anti-ATR antibody from abcam (ab54793) at a dilution of 1:500 and tissues were stained using automated staining platform (Ventana). Envision + polymer (ready to use; Dako) was used as a secondary antibody. Color was developed with 3,3′-diaminobenzidine (DAB) and instant hematoxylin (Shandon) was used for counterstaining.
Al-Ansari M.M, & Aboussekhra A. (2018). ATR suppresses the pro-tumorigenic functions of breast stromal fibroblasts. Oncotarget, 9(78), 34681-34690.
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2

Evaluating ATR Expression in Cancer Tissue

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Immunohistochemistry for ATR was done on formalin-fixed, paraffin-embedded tissue using anti-ATR from Abcam company (ab54793) overnight at a dilution of 1:500, and they were stained using automated staining platform (Ventana). Envision + polymer (ready to use; Dako) was used as a secondary antibody. Color was developed with 3,3′-diaminobenzidine (DAB), and instant hematoxylin (Shandon) was used for counterstaining. The ATR level was evaluated and verified by two qualified pathologists, who scored both the proportion of positive cells as well as the intensity of ATR expression in both cancer cells and their stromal fibroblasts.
Al-Ansari M.M., Al-Saif M., Arafah M., Eldali A.M., Tulbah A., Al-Tweigeri T., Semlali A., Khabar K.S, & Aboussekhra A. (2020). Clinical and functional significance of tumor/stromal ATR expression in breast cancer patients. Breast Cancer Research : BCR, 22, 49.
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3

Western Blot Analysis of Signaling Proteins

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This has been performed as previously described [10 (link)]. Antibodies directed against AUF1 (ab50692) and ATR (ab54793) were purchased from Abcam (Cambridge, MA), STAT3 and pSTAT3-Tyr705 (D3A7) from Cell Signaling (Danvers, MA), and glyceraldehydes-3-phosphate dehydrogenase (GAPDH, FL-335) from Santa Cruz (Santa Cruz, CA).
Al-Ansari M.M., Al-Saif M., Arafah M., Eldali A.M., Tulbah A., Al-Tweigeri T., Semlali A., Khabar K.S, & Aboussekhra A. (2020). Clinical and functional significance of tumor/stromal ATR expression in breast cancer patients. Breast Cancer Research : BCR, 22, 49.
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4

Comprehensive DNA Damage Marker Analysis

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The following antibodies were tested: anti-MLH1 (Abcam #ab251576), anti-MSH2 (Abcam #ab228334), anti-ATM (Abcam #ab224947), anti-phospho ATM (Abcam #ab208775), anti-ATR (Abcam #ab54793), anti-phospho ATR (Abcam #ab230831) anti-PMS2 (Abcam #ab214442), anti-BARD1 (Abcam #ab226854), anti-BRCA2 (Proteintech #67285), anti-BRCA1 (Abcam #ab215988), anti-P53 (Proteintech #60283), and anti-phospho P53 (Abcam #ab223868). Flow cytometry was performed using the strategy that is previously described.9 (link) In brief, cells were partially lysed and treated with antibodies conjugated to fluorochromes. DAPI (Thermo Fisher Scientific #62248) was used as a DNA marker and presence of S6 ribosomal protein (Life Techologies #710405) was used as a marker for intact whole cells. Cell concentration was 100 to 500 cells per μl and antibody concentration was 0.001 μg/μl. Operator was blinded to the genetic background of samples when conducting FVAs.
Alim I., Loke J., Yam S., Templeton A.S., Newcomb P., Lindor N.M., Pai R.K., Jenkins M.A., Buchanan D.D., Gallinger S., Klugman S, & Ostrer H. (2022). Cancer Risk C (CR-C), a functional genomics test is a sensitive and rapid test for germline mismatch repair deficiency. Genetics in medicine : official journal of the American College of Medical Genetics, 24(9), 1821-1830.
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