Anti hmb 45

The antibodies used in this study were anti-ERα (#IR151, Dako), anti-FSCN1 (#SC-56531, Santa Cruz Biotechnology), anti-HMB-45 (#SC-59305, Santa Cruz Biotechnology), anti ID1 (#SC-488, Santa Cruz Biotechnology), anti-PR (#IR168, Dako), anti-phospho-Ser235-236 S6 ribosomal protein (anti-pS6; clone 91B2, Cell Signaling Technology), anti-SMA (#A2547, Sigma-Aldrich), and anti-SOX9 (#AB5535, Millipore).

Cultured normal human/depigmented donor skin tissues were treated with EB (1 mM for a week) at 37 °C and 5% CO₂. The skin tissues were washed thrice with phosphate-buffered saline (PBS), after which they were fixed in 4% (V/V) paraformaldehyde, dehydrated, and embedded in paraffin. The paraffin-embedded tissues were then sectioned into 5-μm-thick slices using a microtome and stained using the Masson-Fontana dye kit (Sbjbio Biotechnology Co., Ltd., Nanjing, China) and anti-HMB45 (referred to as melanoma gp100) (Santa Cruz Biotechnology, Inc., Dallas, TX, USA) according to the manufacturer's instructions. Images were captured using a microscope (BX43F; Olympus Corporation, Tokyo, Japan).
Mouse dorsal skin tissues were obtained, after which they were fixed in 4% (V/V) paraformaldehyde, dehydrated, and embedded in paraffin. The paraffin-embedded skin tissues were then sectioned into 5-μm-thick slices using a microtome and stained using the hematoxylin-eosin (H&E) dye and anti-TYR (Abcam Trading Co., Ltd., Shanghai, China), followed by incubation with the corresponding fluorescent dye-conjugated secondary antibodies (Servicebio Technology Co., Ltd., Wuhan, China), according to the manufacturer's instructions. Images were captured using a microscope (BX43F; Olympus Corporation).