Anti cd86 pe cy5 clone gl1

Manufactured by Thermo Fisher Scientific

Anti-CD86-PE-Cy5 (clone GL1) is a fluorescence-labeled antibody used for flow cytometry. It binds to the CD86 surface protein, which is expressed on antigen-presenting cells. The PE-Cy5 fluorescent dye is conjugated to the antibody, allowing for detection and analysis of CD86-positive cells.

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Lab products found in correlation

Fc blocking antibody, by BD (1 mentions) Mitotracker green fm, by Thermo Fisher Scientific (1 mentions) Mitosox red mitochondrial superoxide indicator, by Thermo Fisher Scientific (1 mentions) Mitotracker red cmxros, by Thermo Fisher Scientific (1 mentions) Ultra low attachment plate, by Corning (1 mentions) Facscelesta, by BD (1 mentions) Gallios 561, by Beckman Coulter (1 mentions) Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Kaluza software, by Beckman Coulter (1 mentions)

Close spelling variants of this product

Anti-CD86 (clone GL1, CD86 (clone GL1), CD86-PE-Cy5 CD86 (GL1, Anti-CD86-PE CD86-PE Anti-CD86

2 protocols using anti cd86 pe cy5 clone gl1

Multiparametric Phenotypic Profiling of Macrophages

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Cells were plated in ultra-low attachment plates (Corning) and cultured
in basal DMEM, pH 6.8 or 7.4, for 24 h. Cells were then treated with Fc blocking
antibody (purified anti-mouse CD16/32, BD) for 15 mins and stained with
anti-F4/80-BV421 (clone BM8, Biolegend), anti-CD206-APC (clone C068C2,
Biolegend), anti-CD301(Mgl1)-PE (clone LOM-14, Biolegend), anti-MHC II-PE-Cy7
(clone M5/114.15.2, Biolegend) and anti-CD86-PE-Cy5 (clone GL1, eBioscience)
antibodies for 30 min on ice in dark. To analyze mitochondria, live cells were
stained with MitoTracker^™ Green FM,
MitoTracker^™ Red CMXRos and MitoSOX^™ Red
Mitochondrial Superoxide Indicator (Invitrogen) to determine mitochondrial mass,
inner membrane potential and reactive oxygen species (ROS) respectively. After
wash, cell fluorescence was measured using flow cytometry (BD FACSCelesta) and
the data were analyzed using Flow Jo software (TreeStar, Inc).

Jiang W., Le J., Wang P.Y., Cheng X., Smelkinson M., Dong W., Yang C., Chu Y., Hwang P.M., Munford R.S, & Lu M. (2021). Extracellular acidity reprograms macrophage metabolism and innate responsiveness. Journal of immunology (Baltimore, Md. : 1950), 206(12), 3021-3031.

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Multiparameter Flow Cytometry Analysis of Podocytes

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Multiparameter flow cytometry (FACS) was performed using a Beckman Coulter Gallios 561 compensated with single fluorochromes and analyzed using Kaluza™ software (Beckman Coulter). Differentiated transformed mouse WT and FcRn KO podocytes populations were identified by their characteristic forward scatter/side scatter (fsc:ssc) properties. Monoclonal anti-mouse antibodies anti-CD80-FITC (clone 16-10A1), anti-MHCII-APC (clone MS/114.15.2), anti-CD86-PE-Cy5 (clone GL1) were purchased from eBiosciences and anti-PE-ICOSL (clone 9F.8A4) was purchased from BioLegend. The Live/Dead Fixable Aqua Dead Cell Stain Kit (Invitrogen) was used to assess the number of live cells. Cells were detached, washed and stained for surface antigen expression at 4°C in the dark for 60 minutes and then washed again prior to flow cytometry. Unstained cells and fluorescence minus one (FMO) samples were used as controls.

Dylewski J.F., Tonsawan P., Garcia G., Lewis L, & Blaine J. (2020). Podocyte-specific knockout of the neonatal Fc receptor (FcRn) results in differential protection depending on the model of glomerulonephritis. PLoS ONE, 15(12), e0230401.

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