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Cgrp antibody

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The CGRP antibody is a laboratory tool used in research applications. It is a monoclonal antibody that specifically binds to the calcitonin gene-related peptide (CGRP), a neuropeptide involved in various physiological processes. The CGRP antibody can be utilized in various experimental techniques, such as immunoassays and immunohistochemistry, to detect and quantify CGRP levels or distribution in biological samples.

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Lab products found in correlation

Rat anti f4 80, by Abcam (2 mentions) Cy3 conjugated anti rabbit igg, by Jackson ImmunoResearch (2 mentions) Cy3 conjugated anti rat igg, by Abcam (2 mentions) Mab360, by Merck Group (1 mentions) Ccd spot camera, by Leica camera (1 mentions) Cy3 or alexa 488 conjugated secondary antibodies, by Jackson ImmunoResearch (1 mentions) Pierce bca assay kit, by Thermo Fisher Scientific (1 mentions) Nupage 4 12 bis tris gel, by Thermo Fisher Scientific (1 mentions) β tubulin, by Merck Group (1 mentions) Nitrocellulose membrane, by Bio-Rad (1 mentions)

3 protocols using cgrp antibody

1

Immunofluorescence Analysis of DRG Nociceptors

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Animals were deeply anesthetized with isoflurane and perfused through the ascending aorta with PBS followed by 4% paraformaldehyde in 0.1 M PB. After the perfusion, the DRGs were removed and postfixed in the same fixative overnight. DRG sections (14 μm) were cut in a cryostat and processed for immunofluorescence as we described previously5 (link). The sections were first blocked with 5% donkey serum for 2 h at room temperature, then incubated overnight at 4 °C with the following primary antibodies: CXCL13 (goat, 1:100, Santa Cruz, sc-8182), CXCR5 (rabbit, 1:100; Santa Cruz, sc-30029), glial fibrillary acidic protein (GFAP, mouse, 1:5000, Millipore, MAB360), peptidergic C-nociceptors marker, calcitonin gene–related peptide (CGRP) antibody (mouse, 1:5,000, Sigma-Aldrich, St. Louis, MO), nonpeptidergic C-nociceptors marker, IB4 (1:50; Sigma-Aldrich), and A fiber afferents marker, NF200 antibody (mouse, 1:500; Millipore). The sections were then incubated for 2 h at room temperature with Cy3- or Alexa 488-conjugated secondary antibodies (1:1000, Jackson ImmunoResearch, West Grove, PA). The stained sections were examined with a Leica fluorescence microscope, and images were captured with a CCD Spot camera.
Wu X.B., Cao D.L., Zhang X., Jiang B.C., Zhao L.X., Qian B, & Gao Y.J. (2016). CXCL13/CXCR5 enhances sodium channel Nav1.8 current density via p38 MAP kinase in primary sensory neurons following inflammatory pain. Scientific Reports, 6, 34836.
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2

Immunohistochemical and Western Blot Analysis of Prostate and Bladder Tissues

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Immunohistochemistry was performed as described previously12 (link), 13 , 14 (link). Sections of prostates or bladder were incubated with rabbit anti-calcitonin gene-related peptide (CGRP) antibody (1:2000; Sigma-Aldrich, St. Louis, MO) followed by Cy3-conjugated anti-rabbit IgG (1:200, Jackson Immunoresearch, West Grove,PA) to immunostain peptidergic afferent fibers. Macrophages were stained in other prostate or bladder tissue sections using rat anti-F4/80 (1:1000; Abcam, Cambridge, MA) and Cy3-conjugated anti-rat IgG (1:200).
In other mice, prostates and bladders were collected after intravascular cold saline perfusion and homogenized; the supernatants were collected and immediately stored at −80°C. Protein concentration was determined by the BCA method (Pierce BCA assay kit, Life Technologies, Grand Island, NY). 40 µg of solubilized proteins were loaded in Nupage Bis-Tris 4–12% gels (Life Technologies). After separation, proteins were transferred electrophoretically to nitrocellulose membranes (Bio-Rad) and western blots were performed for CD4 (1:1000; Novus, Little, CO) and β-tubulin (1:2000; Sigma-Aldrich) for the loading control. Mouse spleen was used as a positive control.
Schwartz E.S., La J.H., Young E.E., Feng B., Joyce S, & Gebhart G.F. (2016). Chronic prostatitis induces urinary bladder hypersensitivity and sensitizes bladder afferents in the mouse. The Journal of urology, 196(3), 892-901.
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3

Immunostaining Prostate Sensory Fibers

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Immunohistochemistry was performed as described previously [30 (link)]. Sections of prostates were incubated with rabbit anti-calcitonin gene-related peptide (CGRP) antibody (1:2000; Sigma-Aldrich) followed by Cy3-conjugated anti-rabbit IgG (1:200, Jackson Immunoresearch, WestGrove, PA) to immunostain peptidergic afferent (sensory) fibers. Monocytes and macrophages were stained in other prostate tissue sections using rat anti-F4/80 (1:1000; Abcam, Cambridge, MA) and Cy3-conjugated anti-rat IgG (1:200).
Schwartz E.S., Xie A., La J.H, & Gebhart G.F. (2015). Nociceptive and Inflammatory Mediator Upregulation in a Mouse Model of Chronic Prostatitis. Pain, 156(8), 1537-1544.
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