Latex beads with a mean particle size of 3 μm (Sigma) were washed with sterile DPBS (Gibco), resuspended in DPBS, and incubated with 1 μg per 107 beads of CD21/CD35 monoclonal antibody (clone 4E3; eBioscience), IgM rat anti-mouse antibody (clone II/41; BD Biosciences), or a combination of both for 2 h at room temperature under continuous mixing on a rotating wheel. As controls, beads were either uncoupled or coupled to the isotype control antibody rat IgG2a kappa isotype control (clone eBR2a; eBioscience). After incubation, the antibody-coupled beads were washed and resuspended in complete IMDM (Gibco, Invitrogen) before incubation with the B cells at an MOI of 7 for 5 h.
Cd21 cd35 monoclonal antibody clone 4e3
Manufactured by Thermo Fisher Scientific
The CD21/CD35 monoclonal antibody (clone 4E3) is a laboratory tool used in flow cytometry and other immunological applications. It binds to the CD21 and CD35 surface proteins expressed on certain immune cells. This antibody can be used to identify and analyze these cell populations.
Automatically generated - may contain errors
Lab products found in correlation
Latex bead, by Merck Group (2 mentions)
Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (2 mentions)
Igm rat anti mouse antibody clone 2 41, by BD (2 mentions)
Rat igg2a kappa isotype control clone ebr2a, by Thermo Fisher Scientific (2 mentions)
Complete imdm, by Thermo Fisher Scientific (2 mentions)
2 protocols using cd21 cd35 monoclonal antibody clone 4e3
1
Latex Bead Conjugation for B cell Activation
2
Latex Bead Conjugation for B cell Activation
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Latex beads with a mean particle size of 3 μm (Sigma) were washed with sterile DPBS (Gibco), resuspended in DPBS, and incubated with 1 μg per 107 beads of CD21/CD35 monoclonal antibody (clone 4E3; eBioscience), IgM rat anti-mouse antibody (clone II/41; BD Biosciences), or a combination of both for 2 h at room temperature under continuous mixing on a rotating wheel. As controls, beads were either uncoupled or coupled to the isotype control antibody rat IgG2a kappa isotype control (clone eBR2a; eBioscience). After incubation, the antibody-coupled beads were washed and resuspended in complete IMDM (Gibco, Invitrogen) before incubation with the B cells at an MOI of 7 for 5 h.
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