Tecnai g2 20 twin transmission

Sf9 cells seed in 35-mm plate (1×10⁶ cells/plate) were transfected with 2.0 μg of vAc^{ac75ko-rep-PH} or vAc^ac75ko-PH. At 48 and 96 hpt, the cells were fixed with 2.5% glutaradehyde, then dislodged and collected by centrifugation at 2,400 rpm for 5 min. The cell pellet was dehydrated, embedded, sectioned, and stained as described previously [9 (link)]. Samples were examined with a FEI Tecnai G²20 TWIN transmission electron microscope at an accelerating voltage of 200 kV.

The size and morphology of nanoparticles were characterized by FEI Tecnai G2 20 TWIN transmission electron microscope (TEM) at 200 kV. TEM samples were prepared by dipping the copper grid into a diluted nanoparticles' solution. Powder X-ray diffraction (XRD) measurements of the nanoparticle samples were conducted on a Bruker D4 diffractometer (Cu Kα radiation, λ = 1.54056 Å) of which the 2θ range is from 10 to 90° and the scanning rate is 0.5°/min. Fourier transform infrared (FTIR) spectra were collected using an IRPRESTIGE-21 spectrometer (Shimadzu). Nanoparticles were mixed with KBr and tableted into pellet to make FTIR samples. E₂ release studies were carried out on Shimadzu UV 2550 spectrometer. The upconversion luminescence (UCL) spectra were collected by using Edinburgh FLS-920 spectrometer equipped with an excitation source of 0-3 W adjustable 980 nm semiconductor laser (Connet Fiber Optics, China). All the photoluminescence studies were carried out at room temperature.

For transmission electron microscopy (TEM) observation, S. aureus ATCC29213 were cultured in MHB to achieve an OD₆₃₀ = 0.2. Then, the bacteria were treated with 1 × MIC peptide for 0.5 h (150 rpm and 37°C). After that, the bacterial cells were harvested (10,000 × g, 4°C, 5 min) and fixed with 2.5% glutaraldehyde (Sigma-Aldrich) (Ma et al., 2016 (link)). The morphologies of the bacteria were observed by using an FEI Tecnai G2 20 TWIN transmission electron microscope.