Anti grp94

Western blot was performed as described previously [14 (link)]. Antibodies for anti-calumenin (1: 500), anti-caspase-3 (1: 500), anti-caspase-9 (1: 500), anti-caspase-12 (1: 500) were obtained from Bioss, anti-CHOP (1: 1000), anti-Bcl-2 (1: 1000) were obtained from Wanleibio, and anti-GRP78 (1: 400), anti-GRP94 (1: 400), anti-Bax (1: 400), anti-ATF-6 (1: 400) were purchased from Boster, and anti-p-PERK (1:750), anti-PERK (1:750), anti-spliced XBP-1 (1:500), anti-unspliced XBP-1 (1:500) were obtained from Abcam. Protein was extracted and mixed in loading buffer, and then equal amounts were fractionated on gel and transferred onto Hybond-C Extra nitrocellulose membrane using a semidry transfer apparatus. Protein was blocked with nonfat dry milk and detected with supersignal west pico chemiluminescent substrate.

Western blot was performed as described previously [13 (link)]. The primary antibodies contain anti-caspase-3 (1:500), anti-caspase-9 (1:500), anti-caspase-12 (1:500) (Bioss), anti-CHOP (1:1000), anti-Bcl-2 (1:1000) (Wanleibio), and anti-GRP78 (1:400), anti-GRP94 (1:400), anti-Bax (1:400) (Boster).