The elemental composition of the dyes was confirmed using an Agilent Technologies 6520 Accurate-Mass QTOF LC/MS (Agilent, Santa Barbara, CA) equipped with an ESI source, operated in negative or positive ion mode. Standard dye solutions were prepared by dissolving 1 mg of dye into 1 mL of methanol : water (60 : 40, v/v). The dye solutions were injected into the ESI source using a Harvard PhD 2000 Infusion syringe pump at a rate of 6 μL min–1. The operating conditions for ion formation consisted of nitrogen drying gas at a temperature of 350 °C and a rate of 10 L min–1, 35 psig nebulizer, 175 V fragmentor voltage, 65 V skimmer voltage, 750 V octopole voltage, 3500 V Vcap voltage, and 0.029 μA capillary current. The data acquisition and qualitative analysis were carried out by Agilent Mass Hunter Workstation Acquisition version B.05.00 and Qualitative Analysis Workstation Software version B.06.00. The UV-Visible spectra of dyes A–C were measured in dimethylformamide using an Agilent Technologies Cary 300 spectrometer.
Cary 300 spectrometer
Manufactured by Agilent Technologies
Sourced in United States
The Cary 300 spectrometer is a versatile laboratory instrument used for spectroscopic analysis. It measures the absorption or transmission of light through a sample over a range of wavelengths. The Cary 300 can be used to identify and quantify various compounds in a sample.
Automatically generated - may contain errors
Lab products found in correlation
Nicolet nexus 470 ft ir spectrometer, by Thermo Fisher Scientific (3 mentions)
Xevo g2 q tof spectrometer, by Waters Corporation (2 mentions)
Avance 400 ft nmr spectrometer, by Bruker (2 mentions)
Sd2000, by OceanOptics (2 mentions)
Cary 670 ftir spectrometer, by Agilent Technologies (1 mentions)
J 815 spectrometer, by Jasco (1 mentions)
2487 dual λ absorbance, by Waters Corporation (1 mentions)
Inova spectrometer, by Agilent Technologies (1 mentions)
Silica gel, by Qingdao Marine Chemical (1 mentions)
600 pump, by Waters Corporation (1 mentions)
Sephadex lh 20, by GE Healthcare (1 mentions)
Avance 600 nmr spectrometer, by Bruker (1 mentions)
Autopol 3 automatic polarimeter, by Rudolph Research Analytical (1 mentions)
J 810 spectropolarimeter, by Jasco (1 mentions)
Eclipse xdb c18, by Agilent Technologies (1 mentions)
Agilent 1260 series, by Agilent Technologies (1 mentions)
Pack ods a column, by YMC (1 mentions)
Fluoview fv10i, by Olympus (1 mentions)
Fluorolog 2, by SPEX (1 mentions)
Unity 400 400 mhz nmr spectrometer, by Agilent Technologies (1 mentions)
14 protocols using cary 300 spectrometer
1
Elemental Composition Analysis of Dyes
2
Characterization of Human iNOS Proteins
3
Spectroscopic Analysis of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Optical rotations were measured on an Autopol III automatic polarimeter (Rudolph Research, Hackettstown, NJ, USA). UV spectra were measured on a Cary 300 spectrometer (Agilent, Melbourne, Australia). ECD spectra were recorded on a J-815 spectrometer (JASCO, Tokyo, Japan). IR spectra were acquired on an Impact 400 FT-IR Spectrophotometer (Nicolet, Madison, WI, USA). Standard pulse sequences were used for all NMR experiments, which were run on either a Bruker spectrometer (600 MHz for 1H or 150 MHz for 13C, Karlsruhe, Germany) or a Varian INOVA spectrometer (500 MHz for 1H or 125 MHz for 13C, Palo Alto, CA, USA) equipped with an inverse detection probe. Residual solvent shifts for acetone-d6 were referenced to δH 2.05, δC 206.7 and 29.9, respectively. Accurate mass measurements were obtained on a Q-Trap LC/MS/MS (Turbo ionspray source) spectrometer (Sciex, Toronto, ON, Canada). Column chromatography (CC) was run using silica gel (200–300 mesh, Qingdao Marine Chemical Inc., Qingdao, China), and Sephadex LH-20 (Pharmacia Biotech AB, Uppsala, Sweden). HPLC separation was done on Waters HPLC components (Milford, MA, USA) comprising of a Waters 600 pump, a Waters 600 controller, a Waters 2487 dual λ absorbance, with GRACE preparative (250 × 19 mm) Rp C18 (5 μm) columns.
4
Analytical Techniques for Natural Product Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Optical rotations were measured on an Autopol III automatic polarimeter (Rudolph Research Analytical, Hackettstown, NJ, USA). UV spectra were collected on a Cary 300 spectrometer (VARIAN, Palo Alto, CA, USA). IR spectra were collected on a Nicolet Nexus 470 FT-IR spectrometer (Thermo Scientific, Waltham, MA, USA). CD spectra were collected on a J-810 spectropolarimeter (Jasco Corporation, Tokyo, Japan). 1H and 13C-NMR spectra were collected on a Bruker Avance-600 NMR spectrometer (Bruker Corporation, Billerica, MA, USA). HRESIMS spectra were collected on a Waters Xevo G2 Q-TOF spectrometer (Waters, Milford, MA, USA). HPLC analysis was performed on an Agilent 1260 series (Agilent Technologies, Santa Clara, CA, USA) with a C18 RP-column (Eclipse XDBC18, 150 × 4.6 mm, 5 μm, Agilent Technologies, Santa Clara, CA, USA). Semi-preparative HPLC was performed on a SSI 23201 system (Scientific Systems Inc., State College, PA, USA) with a YMC-Pack ODS-A column (250 × 10 mm, 5 μm, YMC CO., LTD. Shimogyo-ku, Kyoto, Japan). MPLC was performed on a LC3000 series (Beijing Tong Heng Innovation Technology, Beijing, China) with a ClaricepTM Flash i-series C18 cartridge (20–35 μm, 40 g, Bonna-Agela, Wilmington, DE, USA). Size exclusion chromatography was carried out using a Sephadex LH-20 (GE Healthcare, Chicago, IL, USA) column.
5
Nanoparticle Characterization by Multimodal Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
UV–Vis spectroscopy was performed in Suprasil quartz glass cuvettes with a sample volume of 600 μL with a Varian Cary 300 spectrometer. Differential centrifugal sedimentation was done with a CPS Instruments DC 24,000 disc centrifuge (24,000 rpm). A density gradient was formed with two sucrose solutions (8 wt% and 24 wt%) and capped with 0.5 mL dodecane as stabilizing agent. A poly(vinyl chloride) (PVC) latex in water with a particle size of 483 nm provided by CPS Instruments served as calibration standard. The DCS calibration was carried out prior to each run. A sample volume of 100 μL of dispersed nanoparticles was used. The recording time was about 6 h at the given centrifugation speed. For elemental analysis (gold and copper), the nanoparticles were dissolved in aqua regia and analyzed by atomic absorption spectroscopy (AAS; Thermo Electron M-Series spectrometer; graphite tube furnace according to DIN EN ISO/IEC 17025:2005). Confocal laser scanning microscopy (CLSM) was carried out with an Olympus Fluoview Fv10i (Olympus, Tokyo, Japan) microscope.
6
Spectroscopic Characterization of DNA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
UV/vis absorption spectra over the range 230-500 nm were recorded with a Varian Cary 300 spectrometer. Measurements were performed in a double cuvette (closed but not evacuated) having sections of 1 and 10 mm optical path length. An equivalent cuvette with pure solvent was used for reference. In melting experiments, after each rising temperature step of 5 1C the sample and holder were allowed to equilibrate for 10 minutes. A total concentration c T = 20.5 mM of single strands was estimated. Spectra between 20 and 80 1C were recorded and corrected for density change. Fluorescence spectra at 25 1C were obtained on a Spex Fluorolog-2, by photometric comparison with a secondary standard lamp. Wavelength axes were calibrated with a holmium glass filter or a Hg lamp.
7
UV-Vis Dye Rejection Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
UV-visible (UV-Vis) spectra were recorded in transmission mode using a dual-beam configuration on a Cary 300 spectrometer. Dye rejection was quantified by UV-Vis spectrophotometry of permeate solutions (diluted as necessary) compared with UV-Vis absorbances of calibrated dye standard solutions at the characteristic peak absorbance wavelengths of the solutes.
8
Characterization of 5-(4-aminophenyl)-10,15,20-triphenylporphyrin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
UV-Vis spectra were collected on a Cary 300 spectrometer. IR spectra were recorded on a Nicolet iS50 spectrometer using KBr pellets in the region of 4000–500 cm−1. 1H NMR spectra were recorded on a Varian Unity 400 (400 MHz) NMR spectrometer. Chemical shifts were reported on the d-scale relative to tetramethylsilane (TMS). Mass spectra were obtained using a Brucker Autoflex speed TOF/TOF Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry. Thermal analysis was recorded on Perkin-Elmer TG-7 apparatus (sample: 3–4 mg, heating rate: 10 °C/min, and nitrogen atmosphere). Fluorescence spectra were recorded with a HITACHI F-7000 spectrofluorometer. SEM figures were collected by the HITACHI SU8100 scanning electron microscope. A single-spindle electrostatic spinning machine was used to prepare electrospun fibers.
The reagents and solvents used in the experiment were of the commercial analytical pure grade and were used without further purification. The 5-(4-aminophenyl)-10,15,20-triphenylporphyrin was synthesized in the laboratory according to [21 (link)].
The reagents and solvents used in the experiment were of the commercial analytical pure grade and were used without further purification. The 5-(4-aminophenyl)-10,15,20-triphenylporphyrin was synthesized in the laboratory according to [21 (link)].
9
Analytical Techniques for Chemical Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
UV spectra were acquired with a Cary 300 spectrometer. IR spectra were obtained using a Nicolet 5700FTIR microscope spectrometer. Analytical HPLC was conducted on an Agilent system with a 1260 Quat-Pump and DAD detector. For semi-preparative HPLC, a reverse-phase C18 column (Spursil 5μm C18 column: 250 × 10.0 mm) was used with MeCN-H2O as a solvent system. LC-MS was performed on a 1100–6410 Triple Quad from Agilent or an Agilent 1100 LC/MSD with a G1946D single quadrupole mass spectrometer. High-resolution mass spectrometry was carried out on a XEVO G2-XS QTof from Waters. NMR data were collected using a Bruker-600 or an ADVANCE HD 800 MHz and a Bruker Avance Ⅲ HD 700 MHz spectrometer, where chemical shifts (δ) were reported in ppm and referenced to DMSO-d6 solvent signal (δH 2.49 and δC 39.5), CDCl3 solvent signal (δH 7.26 and δC 77.0) and acetone-d6 solvent signal (δH 2.04 and δC 206.0). 3T3-L1 fibroblast cell (pre-adipocyte) line was purchased from the Cell Center of the Institute of Basic Medicine, Chinese Academy of Medical Sciences.
10
Luminescence Quantum Yield Determination
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
UV–Vis spectra in dichloromethane solutions were recorded on an Agilent Cary 300 spectrometer (Santa Clara, CA, USA), for the fluorescence spectra, a Cary Eclipse spectrofluorometer has been used. All measured luminescence spectra were corrected for nonuniformity of detector spectral sensitivity. Rhodamine 6G (φfl 0.95) in ethanol was used as a reference for the luminescence quantum yield measurements. The luminescence quantum yields were calculated using equation:
where φi and φ0 are the luminescence quantum yields of the studied solution and the standard compound, respectively; Ai and A0 are the absorptions of the studied solution and the standard, respectively; Si and S0 are the areas underneath the curves of the luminescence spectra of the studied solution and the standard, respectively; and ni and n0 are the refractive indices of the solvents for the substance under study and the standard compound (ni 1.4242, DCM; n0 1.361, EtOH).
where φi and φ0 are the luminescence quantum yields of the studied solution and the standard compound, respectively; Ai and A0 are the absorptions of the studied solution and the standard, respectively; Si and S0 are the areas underneath the curves of the luminescence spectra of the studied solution and the standard, respectively; and ni and n0 are the refractive indices of the solvents for the substance under study and the standard compound (ni 1.4242, DCM; n0 1.361, EtOH).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!