Monoclonal anti ptbp2

Manufactured by Abnova

Sourced in United States

Monoclonal anti-PTBP2 is a laboratory reagent used for the detection and study of the PTBP2 protein. It is a purified antibody that specifically binds to the PTBP2 protein, allowing researchers to identify and quantify its presence in biological samples.

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Lab products found in correlation

Monoclonal anti flag m2, by Merck Group (2 mentions) Monoclonal anti nova1, by Abnova (2 mentions) Ecl system, by Merck Group (2 mentions) Las 4000 imaging system, by Fujifilm (2 mentions) Monoclonal anti α tubulin, by Abcam (1 mentions) Monoclonal anti e cadherin, by Abcam (1 mentions) Polyclonal anti rbm4, by Santa Cruz Biotechnology (1 mentions) Polyclonal anti erk1 2, by Cell Signaling Technology (1 mentions)

2 protocols using monoclonal anti ptbp2

Immunoblot Analysis Protocol for Protein Detection

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The immunoblot analysis was performed using an enhanced chemiluminescence (ECL) system (Millipore, Billerica, MA, USA), and results were monitored using the LAS-4000 imaging system (Fujifilm, Tokyo, Japan). Primary antibodies used in this study included polyclonal anti-RBM416 (link), monoclonal anti-PTBP2 (Abnova, Taipei, Taiwan), monoclonal anti-SRSF6 (EMD, Millipore), monoclonal anti-Nova1 (Abnova), monoclonal anti-actin (Millipore), monoclonal anti-α-tubulin (Abcam, Cambridge, UK), monoclonal anti-E-cadherin (Abcam), monoclonal anti-N-cadherin (Abcam), and monoclonal anti-FLAG M2 (Sigma-Aldrich, St. Louis, MO, USA). Signal intensities were evaluated using TotalLab Quant Software (where available?).

Lin J.C., Lee Y.C., Liang Y.C., Fann Y.C., Johnson K.R, & Lin Y.J. (2017). The impact of the RBM4-initiated splicing cascade on modulating the carcinogenic signature of colorectal cancer cells. Scientific Reports, 7, 44204.

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Quantitative Immunoblot Analysis Techniques

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The immunoblot analysis was conducted using an enhanced chemiluminescence (ECL) system (Millipore, Billerica, MA, USA), and images were analyzed with the LAS-4000 imaging system (Fujifilm, Tokyo, Japan). Primary antibodies used in this study included polyclonal anti-RBM4 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), monoclonal anti- PTBP2 (Abnova, Taipei, Taiwan), monoclonal anti-PTBP1 (EMD, Millipore), monoclonal anti-Nova1(Abnova), monoclonal anti-Actin (EMD, Millipore), monoclonal anti-FLAG M2 (Sigma-Aldrich, St. Louis, MO, USA), polyclonal anti-ERK1/2, and monoclonal anti-phospho-ERK1/2 (Cell Signaling Technology, Danvers, MA, USA). Intensities of the detected signals were determined using TotalLab Quant Software.

Lin J.C., Lu Y.H., Liu Y.R, & Lin Y.J. (2016). RBM4a-regulated splicing cascade modulates the differentiation and metabolic activities of brown adipocytes. Scientific Reports, 6, 20665.

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