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Secondary antibody against rabbit igg

Manufactured by Santa Cruz Biotechnology
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Sourced in United States, United Kingdom

Secondary antibody against rabbit IgG. This product is a purified antibody that binds to rabbit immunoglobulin G (IgG) antibodies. It is designed for use in various immunoassay techniques.

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Lab products found in correlation

β actin, by Bioss Antibodies (1 mentions) Gpx4 1 800 antibody, by ABclonal (1 mentions) Tyrosine hydroxylase, by Merck Group (1 mentions) Mptp hcl, by Merck Group (1 mentions) Ecl plus, by Applygen (1 mentions) Pvdf membrane, by Merck Group (1 mentions)

4 protocols using secondary antibody against rabbit igg

1

Cerebellum Tissue Protein Extraction and Quantification

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Protein samples were extracted from the cerebellum tissues and quantified using commercially available kits (Beyotime institute of biotechnology, P.R. China). Detailed treatment methods were performed as previously described (Zhang et al. 2019 (link)). MTF1 (1:700) antibody was purchased from Nan-Jing AnYan Biotechnology Co., Ltd; SepSecS (1:500) antibody was synthesized from Jin-Long Li Lab; GPX4 (1:800) antibody was purchased from Abclonal, USA; β-actin (1:1000, Beijing Biosynthesis Biotechnology Co., Ltd); and secondary antibody against rabbit IgG was purchased from Santa Cruz, CA.
Bi S.S., Talukder M., Jin H.T., Lv M.W., Ge J., Zhang C, & Li J.L. (2022). Cadmium Through Disturbing MTF1-Mediated Metal Response Induced Cerebellar Injury. Neurotoxicity Research, 40(5), 1127-1137.
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2

Colon Mucosal Protein Expression

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Total protein of mucosa in the distal colon and cells was extracted using RIPA lysis buffer and then separated by 10% SDS/PAGE. The separated proteins were transferred onto PVDF membranes, which were then washed for 10 min with TBS and blocked with 5% non-fat dry milk in TBST for 2 h at 25 °C. The blot was incubated with a polyclonal primary antibody against ZO-1, Occludin, Claudin-2, and Nrf2 (Abcam) overnight at 4 °C. After washing in TBST (10 min, three times), the blot was incubated with a secondary antibody against rabbit IgG (Santa Cruz) for 1 h at 25 °C. The blot was finally washed with TBST (10 min, three times), and the protein bands were visualized with a chemiluminescence system. The resulting image was analysed using Image J software.
Li Z.Y., Lin L.H., Liang H.J., Li Y.Q., Zhao F.Q., Sun T.Y., Liu Z.Y., Zhu J.Y., Gu F., Xu J.N., Hao Q.Y., Zhou D.S, & Zhai H.H. (2023). Lycium barbarum polysaccharide alleviates DSS-induced chronic ulcerative colitis by restoring intestinal barrier function and modulating gut microbiota. Annals of Medicine, 55(2), 2290213.
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3

RAGE and COX2 Antibody Validation

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Unless otherwise indicated, all the antibodies in this experiment were purchased from Cell Signal Technology (Hertfordshire, England). Primary antibodies against RAGE and COX2 were supplied by Abcam (Cambridge, United Kingdom) and secondary antibody against rabbit IgG was supplied by Santa Cruz Biotechnology (Santa Cruz, CA, United States). Polyclonal antibodies tyrosine hydroxylase (TH) were purchased from Millipore (Bedford, MA, United States) and Alexa Fluor® 555-conjugated secondary goat anti-rabbit antibody was purchased from Life Technologies (United States). Lentivirus small interference RAGE and the negative control lentiviral vectors were purchased from Shanghai GeneChem (Shanghai, China). MPTP⋅HCl was purchased from Sigma Chemical (St. Louis, MO, United States). MPTP⋅HCl was dissolved in physiological saline (0.9%) at a concentration of 6.0 mg/ml.
Wang X., Sun X., Niu M., Zhang X., Wang J., Zhou C, & Xie A. (2020). RAGE Silencing Ameliorates Neuroinflammation by Inhibition of p38-NF-κB Signaling Pathway in Mouse Model of Parkinson’s Disease. Frontiers in Neuroscience, 14, 353.
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4

Western Blot Analysis of GAP43 Protein

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The proteins (20 μg) were separated by 8% SDS/PAGE and then electroblotted onto a PVDF membrane (Millipore), which was then washed for 10 min with TBST and immersed in blocking buffer containing 5% nonfat dry milk in TBST for 1 h at 25 °C. The blot was washed with TBST and then incubated with a rabbit polyclonal primary GAP43 antibody (Abcam, 1:5000 ab75810) overnight at 4 °C. After the blot was washed in TBST, it was incubated with a secondary antibody against rabbit IgG (Santa Cruz 1:2000) for 1 h at 25 °C. The blot was finally washed with TBST, and the protein bands were visualized with a chemiluminescence system (ECLPlus, Applygen Technologies, Inc.).
Zhang B., Xue H., Wang W., Chen T., Su M., Kang N., Yang J., Bian Z., Wang F, & Tang X. (2020). Comparative proteomic analysis of the brain and colon in three rat models of irritable bowel syndrome. Proteome Science, 18, 1.
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