The human malignant rhabdoid tumor cell line JMU-RTK-2 was obtained from the JCRB cell bank and cultured in Dulbecco’s modified Eagle’s medium supplemented with 5% fetal bovine serum. The rhabdomyosarcoma cell line RMS-YM was obtained from RIKEN BRC cell bank and cultured in RPMI-1640 supplemented with 10% fetal bovine serum, 20 mM-HEPES, and 0.1 mM None-essential amino acids (FUJIFILM Wako Pure Chemical Corporation). All the cells were maintained at 37 °C in a humidified atmosphere containing 5% CO2. Topoisomerase inhibitors, etoposide, doxorubicin, and epirubicin were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan), ellipticine was purchased from Merck (DS, Darmstadt, Germany), topotecan hydrochloride hydrate was purchased from Sigma Aldrich (St Louis, MO, USA), and siremadlin was purchased from Medchemexpress (Monmouth Junction, NJ, USA).
Epirubicin
Manufactured by Fujifilm
Sourced in Japan
Epirubicin is a chemotherapy drug used in the treatment of various types of cancer. It is a synthetic derivative of the natural antibiotic daunorubicin. Epirubicin works by interfering with the growth and division of cancer cells, ultimately leading to their death.
Automatically generated - may contain errors
Lab products found in correlation
Doxorubicin, by Fujifilm (1 mentions)
Etoposide, by Fujifilm (1 mentions)
Topotecan hydrochloride hydrate, by Merck Group (1 mentions)
Rms ym, by RIKEN BioResource Center (1 mentions)
Rpmi 1640, by Fujifilm (1 mentions)
Siremadlin, by MedChemExpress (1 mentions)
5 fluorouracil 5 fu, by Fujifilm (1 mentions)
Docetaxel, by Fujifilm (1 mentions)
Dimethyl sulfoxide (dmso), by Fujifilm (1 mentions)
3 protocols using epirubicin
1
Rhabdoid Tumor and Rhabdomyosarcoma Cell Culture
2
Liver Cancer Cell Line Treatment
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The human liver cancer cell lines HuH1 and HuH7 were obtained from the Japanese Collection of Research Bioresources (Osaka, Japan) and routinely cultured with Dulbecco's modified Eagle's medium supplemented with 10% FBS. Epirubicin and 5-fluorouracil (5-FU) were obtained from Wako Pure Chemical Industries, Ltd. (Tokyo, Japan) and Kyowa Kirin (Tokyo, Japan), respectively. HuH1 and HuH7 cell lines were seeded at 10,000 cells per well in a 6-well plate treated with Epirubicin (0.5 μg/ml for HuH1 and 0.1 μg/ml for HuH7) or 5-FU (2.0 μg/ml for HuH1 and 2.5 μg/ml for HuH7) for 5 days based on IC50 data obtained from a cell proliferation assay. These cells were then used for quantitative reverse transcription-polymerase chain reaction (qRT-PCR), fluorescence-activated cell sorting (FACS), and immunofluorescence analyses, as described previously [12] (link).
3
Breast Cancer Cell Cytotoxicity Assay
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Breast carcinoma cells were cultured in 96-well plates for 24 h, at which point we added epirubicin (Wako, Tokyo, Japan), docetaxel (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), or dimethyl sulfoxide (DMSO) (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) as a control. After 48 h, the cell number was measured as previously reported42 (link).
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