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Indiko clinical chemistry analyzer

Manufactured by Thermo Fisher Scientific
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Sourced in Finland

The Indiko Clinical Chemistry Analyzer is a diagnostic instrument designed for automated analysis of clinical chemistry samples. It is capable of performing a variety of biochemical tests to aid in the diagnosis and monitoring of various health conditions.

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Lab products found in correlation

Vicor2 multilabel counter, by PerkinElmer (1 mentions) Quantikine enzyme linked immunosorbent assay elisa kit, by R&D Systems (1 mentions) Human magnetic luminex assay, by R&D Systems (1 mentions) Bio plex manager software, by Bio-Rad (1 mentions) Bio plex 200, by Bio-Rad (1 mentions) Glucose god fs kit, by DiaSys (1 mentions) Celltiter glo, by Promega (1 mentions) D glucose, by Merck Group (1 mentions) Cholesterol, by Thermo Fisher Scientific (1 mentions)

8 protocols using indiko clinical chemistry analyzer

1

Quantification of Plasma Lipopolysaccharide

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LPS was quantifieded by liquid chromatography coupled with mass spectrometry. This method relies on the quantification of 3-hydroxymyristate (3HM, a fatty acid specific for the lipid A moiety of LPS). This method allows the complete quantification of LPS concentrations in plasma, as compared with an activity assay, which has the potential to omit a part of plasmatic LPS (i.e., inactive/neutralized LPS). The coefficient of variation of the method is 14% (20 (link)). All samples were processed from the same batch. In a cohort of 49 control subjects, the reported median 3HM concentration was 96 [77;116] pmol/ml (12 (link)).
Phospholipid transfer protein (PLTP) activity was measured using a commercially available fluorescence activity assay (Roar Biomedical Inc., New York) according to the manufacturer's instructions. Fluorescence measurements were performed over time on a Vicor2 multilabel counter (Perkin Elmer). Phospholipid transfer activity was calculated from the slope of fluorescence increase between 1 and 30 min.
Total cholesterol, LDL-cholesterol, HDL-cholesterol and triglyceride plasma concentrations were measured by commercially available enzymatic assays using the Thermo Fisher Scientific Indiko Clinical Chemistry Analyzer (Thermo Fisher).
Nguyen M., Gautier T., Reocreux G., Pallot G., Maquart G., Bahr P.A., Tavernier A., Grober J., Masson D., Bouhemad B, & Guinot P.G. (2021). Increased Phospholipid Transfer Protein Activity Is Associated With Markers of Enhanced Lipopolysaccharide Clearance in Human During Cardiopulmonary Bypass. Frontiers in Cardiovascular Medicine, 8, 756269.
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2

Liver Biochemical and Collagen Analyses

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Plasma parameters were determined with an automatic biochemical analyzer (Indiko Clinical Chemistry Analyzer, Thermofisher). Liver triglyceride levels were measured using a commercially available kit (Roche Diagnostics) after homogenization and extraction as described.49 (link) For hydroxyproline levels, liver samples are homogenized in 6 M HCl and hydrolyzed to degrade collagen. Samples are centrifuged and the hydroxyproline content is measured in duplicates in the supernatant, using a colorimetric assay (Quickzyme Biosciences) according to the manufacturer’s instructions.
Seedorf K., Weber C., Vinson C., Berger S., Vuillard L.M., Kiss A., Creusot S., Broux O., Geant A., Ilic C., Lemaitre K., Richard J., Hammoutene A., Mahieux J., Martiny V., Durand D., Melchiore F., Nyerges M., Paradis V., Provost N., Duvivier V, & Delerive P. (2022). Selective disruption of NRF2-KEAP1 interaction leads to NASH resolution and reduction of liver fibrosis in mice. JHEP Reports, 5(4), 100651.
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3

Measurement of Soluble Biomarkers in Plasma

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Soluble CD14 was measured in plasma samples using Quantikine enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems, Minneapolis, MN) according to the manufacturer’s recommendations. Plasma lipoproteins were assayed using commercially available kits (Cholesterol and HDL-cholesterol, Thermo Fisher Scientific, Finland) on an Indiko Clinical Chemistry analyzer (Thermo Fisher Scientific, Finland) according to the manufacturer’s instructions. Phospholipid transfer protein (PLTP) activity was measured in serum using a commercially available fluorescence activity assay from Roar Biomedical (New York, NY, USA), according to the manufacturer’s instructions. The concentrations of tumor necrosis factor α (TNF-α), interleukin (IL)-1β, IL-6 and IL-10 were determined in plasma samples by using a Human Magnetic Luminex assay (R&D Systems, Minneapolis, MN USA) according to the manufacturer’s instructions. Standards and samples were analyzed on a LuminexR® apparatus (Bio-Plex 200, BioRad, München, Germany) using the BioPlex Manager Software (Version 5, BioRad, Hercules, CA).
Adda-Rezig H., Carron C., Pais de Barros J.P., Choubley H., Charron É., Rérole A.L., Laheurte C., Louvat P., Gaiffe É., Simula-Faivre D., Deckert V., Lagrost L., Saas P., Ducloux D, & Bamoulid J. (2021). New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response. Frontiers in Immunology, 12, 658404.
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4

Lipid Extraction and Characterization of Extracellular Vesicles

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We used Folch’s method to purify the lipid sub-fraction. Briefly, EVs were reconstituted in NaCl2, CHCl3 and methanol. After agitation for 2 h, NaCl2 was added, agitated for 2 min, left at room temperature for 5 min and centrifuged for 5 min. The upper phase was aspirated and the lower phase transferred to a Pyrex vial for drying. The lipid sub-fraction was reconstituted in DMSO and micelles, obtained by diluting lipid fraction tenfold in PBS1× at room temperature for 20 min, were applied to Lin− cells for 24 h. For quantification of cholesterol, we used the Indiko Clinical Chemistry Analyzer (Thermo Fisher Scientific). For the glucose oxidase assay, we used the Glucose GOD FS kit (DiaSys Diagnostic Systems GmbH, Holzheim, Germany) and D-( + )-Glucose (Merck) as the standard for quantification. ATP was measured with the Cell Titer Glo (Promega, Charbonnières-les-Bains, France) and standard (A1852, Merck) for the quantification.
Georgievski A., Michel A., Thomas C., Mlamla Z., Pais de Barros J.P., Lemaire-Ewing S., Garrido C, & Quéré R. (2022). Acute lymphoblastic leukemia-derived extracellular vesicles affect quiescence of hematopoietic stem and progenitor cells. Cell Death & Disease, 13(4), 337.
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5

Measuring Serum PLTP Activity in RTR

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PLTP activity was measured in serum of 89 RTR using a commercially available fluorescence activity assay from Roar Biomedical (New York, NY, USA), according to the manufacturer's instructions. This fluorimetric assay measures the transfer (unquenching) of fluorescent phospholipids from donor to acceptor synthetic liposomes. Phospholipid transfer rates were calculated using the initial slope of the phospholipid transfer curve, and were expressed as initial phospholipid transfer rate (i.e., nmol/h/ml serum). Serum lipoproteins were assayed in 89 RTR using commercially available kits (Cholesterol, HDL-Cholesterol and Triglycerides, Thermo Fisher Scientific, Finland) on an Indiko Clinical Chemistry analyzer (Thermo Fisher Scientific, Finland) according to the manufacturer's instructions.
Carron C., Pais de Barros J.P., Gaiffe E., Deckert V., Adda-Rezig H., Roubiou C., Laheurte C., Masson D., Simula-Faivre D., Louvat P., Moulin B., Frimat L., Rieu P., Mousson C., Durrbach A., Heng A.E., Saas P., Ducloux D., Lagrost L, & Bamoulid J. (2019). End-Stage Renal Disease-Associated Gut Bacterial Translocation: Evolution and Impact on Chronic Inflammation and Acute Rejection After Renal Transplantation. Frontiers in Immunology, 10, 1630.
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6

Measuring Liver Enzymes in Plasma

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Plasma levels of ALT and AST were determined with an automatic biochemical analyzer (Indiko Clinical Chemistry Analyzer, Thermofisher).
Sansilvestri Morel P., Duvivier V., Bertin F., Provost N., Hammoutene A., Hubert E.L., Gonzalez A., Tupinon-Mathieu I., Paradis V, & Delerive P. (2022). Procollagen C-Proteinase Enhancer-1 (PCPE-1) deficiency in mice reduces liver fibrosis but not NASH progression. PLoS ONE, 17(2), e0263828.
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7

Serum Biomarker Measurement Protocol

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Serum triglyceride (TG), total cholesterol (TC), serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured using an Thermo Scientific™ Indiko™ Clinical Chemistry Analyzer (Thermo Fisher Scientific, Waltham, USA) according to the manufacturer's instructions.
Wang J., Guo Y., He Y., Qin Y., Li X., Yang L., Liu K, & Xiao L. (2024). Hepatic regulator of G protein signaling 14 ameliorates NAFLD through activating cAMP-AMPK signaling by targeting Giα1/3. Molecular Metabolism, 80, 101882.
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8

Urine Specific Gravity Measurement

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The urine was thawed at room temperature. The urine specific gravity (SG) was measured with a hand held refractometer (RHC-200), calibrated with distilled water. The creatinine concentration of each sample was determined by an enzymatic method on an Indiko Clinical Chemistry Analyzer (Thermo Scientific) using the manufacturer's kit and instructions.
Tordiffe A.S., van Reenen M., Reyers F, & Mienie L.J. (2017). Gas chromatography-mass spectrometry profiles of urinary organic acids in healthy captive cheetahs (Acinonyx jubatus). Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1049-1050.
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