Ht1376

Human bladder cancer cell lines UM-UC-3, T24, HT1376, and 5637 were provided by American Type Culture Collection. UM-UC-3 and HT1376 lines were cultured in Eagle's minimum essential medium (EMEM; Wako), 5637 cells in RPMI1640 (Gibco, Thermo Fisher Scientific, Inc.), and T24 cells in McCoy's 5A medium (Gibco, Thermo Fisher Scientific, Inc.). In all the cultures, the medium was supplemented with streptomycin (100 mg/ml) and penicillin (100 units/ml; Pen strep; Gibco, Thermo Fisher Scientific) as well as with 10% fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Tokyo, Japan). Cells were maintained in a humidified incubator with 5% CO₂ at 37°C.
We purchased 7-week-old female C.B-17/IcrHsd-Prkdcscid mice from Japan SLC (Hamamatsu, Japan). These animals were transferred to a temperature-controlled (20–26°C) and humidity-controlled (40–60%) room with a 12-h light/12-h dark cycle during the experimental period. All animal experiments were approved by the FUJIFILM Animal Experimentation Committee.

The human bladder cancer cell lines (5637, HT1376, UMUC3, TCCSUP and T24) and prostate cancer cell lines (LNCaP and PC-3) were purchased from the American Tissue Culture Collection (Manassas, VA, USA). Cells were authenticated by short tandem repeat analysis performed by Takara Bio, Inc. (Otsu, Japan) and tested to ensure that they were mycoplasma-free by DDC Medical (Thermo Fisher Scientific, Inc., Waltham, MA, USA) in November 2017. Cells were maintained as monolayer cultures at 37°C and 5% CO₂. The 5637 cell line was grown in RPMI-1640 (Sigma-Aldrich; Merck KGaA) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich; Merck KGaA), 1% HEPES, and 1% D-Glucose. HT-1376 was grown in Eagle's minimal essential medium (EMEM; Wako Pure Chemical Industries, Ltd., Osaka, Japan) supplemented with 10% FBS, 1% sodium pyruvate solution (Sigma-Aldrich; Merck KGaA) and 1% MEM non-essential amino acid solution (Thermo Fisher Scientific, Inc.). UMUC3 and TCCSUP were grown in EMEM, LNCaP and PC-3 in RPMI 1640, and T24 in McCoy's 5A medium (Thermo Fisher Scientific, Inc.), supplemented with 10% FBS.