Paraplast tissue embedding medium

All of the liver tissues for the histological chemistry experiment were fixed in 10% neutrally buffered formalin solution at room temperature, dehydrated, and embedded in paraffin (Paraplast Tissue Embedding Medium, LEICA, Buffalo Grove, IL, USA) using a modular tissue embedding system (LEICA EG1150 H, Buffalo Grove, IL, USA). Hematoxylin–eosin (HE) staining was carried out on 5 μm sections using a fully automated rotary microtome (LEICA RM2255, Nussloch, Germany) and mounted onto positive charged slides (Adhesion Microscope Slides, CITOGLAS, Shanghai, China). HE staining of the liver paraffin sections was performed using an HE staining kit (Boster Biological Technology Company, Pleasanton, California, USA) and was pictured by microscope (LEICA DM500, Wetzlar, Germany) with a supporting camera (LEICA ICC50 W, Wetzlar, Germany). The hepatic adipose infiltration cells were counted manually using ImageJ software (Version 1.51q).

Briefly, the fixed liver tissues were dehydrated and embedded in paraffin (Paraplast Tissue Embedding Medium, LEICA, USA) using modular tissue embedding system (LEICA EG1150 H, USA). The 5 μm sections were cut by using fully automated rotary microtome (LEICA RM2255, USA) and mounted in positive charged slides (Adhesion Microscope Slides, CITOGLAS, China). Hematoxylin-eosin (HE) staining was carried out using HE staining kit (Boster Biological Technology Company, China). All the images were acquired by microscope (LEICA DM500, USA) at 200X. Hepatic adipose infiltration cells were counted manually using Image J software.

All the liver tissues fixed in 10% neutrally buffered formalin solution were dehydrated and embedded in paraffin (Paraplast Tissue Embedding Medium, LEICA, Buffalo Grove, IL, USA) using a modular tissue embedding system (LEICA EG1150 H, Buffalo Grove, IL, USA). The embedded tissues were cut into 5 μm sections using a fully automated rotary microtome (LEICA RM2255, Nussloch, Germany), and mounted onto positively charged slides (CITOGLAS, Shanghai, China). HE staining was performed using a HE staining kit (Boster Biological Technology Company, Pleasanton, California, USA) and was imaged by microscope (LEICA DM500, Wetzlar, Germany) using a supporting camera (LEICA ICC50 W, Wetzlar, Germany). The hepatic adipose infiltration cells were counted manually using Image J software (National Institutes of Health, Bethesda, MD, USA).