Anti flag epitope m2

Manufactured by Merck Group

The Anti-Flag Epitope (M2) is a laboratory tool used for the detection and purification of recombinant proteins. It functions as a small peptide tag that can be fused to a protein of interest, allowing for its identification and isolation using specific antibodies.

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Lab products found in correlation

Irdye 800cw donkey anti rabbit, by LI COR (2 mentions) Irdye 800cw donkey anti mouse, by LI COR (2 mentions) Irdye 680cw donkey anti rabbit, by LI COR (2 mentions) Irdye680cw donkey anti mouse, by LI COR (2 mentions) Cdc20, by Santa Cruz Biotechnology (2 mentions) Cdc20 a301 180a, by Fortis Life Sciences (2 mentions) Bubr1, by BD (2 mentions) Anti myc epitope, by Santa Cruz Biotechnology (2 mentions) Anti gfp clone 3 1 and 7, by Roche (2 mentions) Nanodrop, by Thermo Fisher Scientific (2 mentions) Enhanced chemiluminescence, by PerkinElmer (1 mentions)

Close spelling variants of this product

Mouse monoclonal anti-FLAG epitope (clone M2, Mouse anti-FLAG epitope (clone M2, Anti-Flag epitope Anti-FLAG M2 FLAG epitope FLAG M2 Anti-FLAG

3 protocols using anti flag epitope m2

Mitotic Spindle Checkpoint Protein Detection

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The following antibodies were used at the indicated dilutions. CDC20 (sc-13162, Santa Cruz Biotechnology) 1:500; CDC20 (A301-180A, Bethyl laboratories) 1:500; BUBR1 (612503, BD transduction laboratories); BUBR1 (A300-386A, Bethyl laboratories) 1:500; MAD2 (610679, BD transduction laboratories) 1:500; MAD2 (A300-301A, Bethyl Laboratories) 1:500; BUB3 (611730, BD Transduction Laboratories) 1:500; APC3 (610455, BD Transduction Laboratories) 1:500; APC4 (monoclonal antibody raised against a C-terminal peptide) 1:500; BLINKIN (a kind gift from M. Yanagida and T. Kiyomitsu) 1:50; anti-myc-epitope (9E10, Santa Cruz Biotechnology) 1:500; anti-flag epitope (M2, Sigma) 1:5000; anti-GFP (Clone 3.1 and 7.1, Roche) 1:200.
Secondary antibodies: IRDye 680CW donkey anti mouse (926-68072, LI-COR), IRDye 800CW donkey anti mouse (926-32212, LI-COR); IRDye 680CW donkey anti rabbit (926-32223, LI-COR); IRDye 800CW donkey anti rabbit (926-32213, LI-COR) were all used at 1:10000.

Izawa D, & Pines J. (2014). The Mitotic Checkpoint Complex binds a second CDC20 to inhibit active APC/C. Nature, 517(7536), 631-634.

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Mitotic Spindle Checkpoint Protein Detection

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Izawa D, & Pines J. (2014). The Mitotic Checkpoint Complex binds a second CDC20 to inhibit active APC/C. Nature, 517(7536), 631-634.

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Western Blot Analysis of Protein Expression

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Whole cell extracts were prepared by direct lysis of cells in NuPAGE LDS sample buffer. Total protein concentrations were determined by UV absorbance at 280 nm using a Nanodrop (Thermo Scientific) and equal amounts of total protein (30 μg) was resolved on a 10% Bis-Tris NuPAGE gel in 1 × MOPS buffer and subsequently transferred onto PVDF membrane. Following transfer, membranes were probed with the indicated antibodies, and visualized using enhanced chemiluminescence (Perkin Elmer). The antibodies used were anti-FLAG epitope (M2, Sigma, 1:5,000) and anti-eEF2 (#2332, Cell Signaling, 1:1,000). Full uncropped images are provided in Supplementary Fig. 6.

Malina A., Cameron C.J., Robert F., Blanchette M., Dostie J, & Pelletier J. (2015). PAM multiplicity marks genomic target sites as inhibitory to CRISPR-Cas9 editing. Nature Communications, 6, 10124.

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