Il 7rα pe

Manufactured by Thermo Fisher Scientific

IL-7Rα-PE is a fluorochrome-conjugated antibody that binds to the IL-7Rα (CD127) receptor on the surface of cells. It is used for the detection and analysis of IL-7Rα-positive cells in flow cytometry applications.

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IL-7Rα (2 citations)

2 protocols using il 7rα pe

Multiparameter Flow Cytometry Analysis

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Splenocytes and mesenteric lymph node cells were isolated and then stained with anti-mouse CD4-APC, CD8-PerCP-Cy5.5, CD19-PE, CD44-PE, CD62L-FITC, CD69-FITC, IL-7Rα-PE, CD138-APC, PSGL-1-APC, CXCR5-PE, PD-1-FITC, GL-7-FITC, CD95-PE-Cy7, B220-Alexa647 and NK1.1-FITC (eBioscience, San Diego, CA) antibodies for 15 min at 4°C. To assess intracellular cytokine levels, cells were differentiated for 5 days and then re-stimulated with Cell Stimulation Cocktail plus Protein Transport Inhibitors reagent (eBioscience) for 5 h. Then, the cells were fixed, permeabilized, and stained with anti-mouse IFN-γ-FITC, IL-4-PE, IL-17-APC, and IL-9-APC antibodies. Intracellular Foxp3 staining was performed using the Foxp3 Staining Kit (eBioscience). To determine the purity of isolated CD4⁺CD25⁻ T cells, these cells were stained with anti-CD4 and anti-CD25, and followed by FACS analysis.

Park H.J., Kim D.H., Choi J.Y., Kim W.J., Kim J.Y., Senejani A.G., Hwang S.S., Kim L.K., Tobiasova Z., Lee G.R., Craft J., Bothwell A.L, & Choi J.M. (2014). PPARγ Negatively Regulates T Cell Activation to Prevent Follicular Helper T Cells and Germinal Center Formation. PLoS ONE, 9(6), e99127.

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Flow Cytometry Antibody Panel

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Antibodies for flow cytometry were as follows. Anti-mouse antibodies used were: CD93-PE-Cy7 or Biotin (AA4.1), Mac1/CD11b-PE or FITC (M1/70), Gr1-PE (RB6-8C5), B220-PE (RA3-6B2), F4/80-PE (BM8), NK1.1-PE (PK136), TER119-PE (TER-119), IL-7Rα-PE (SB14), CD3-PE (145-2C12), CD4-PE (GK1.5), CD8-PE (53-6.7), CD117-APC (2B8), Sca1-PE-Cy5 (D7), CD16/32-PE-Cy7 (2.4G3) and CD34-FITC (RAM34) (all from eBioscience). The lineage cocktail included the following mAbs: Mac1, Gr1, B220, TER119, IL-7Rα, NK1.1, CD3, CD4, and CD8. Anti-human antibodies were: CD34-APC (8G12) (BD Biosciences), CD38-PE-Cy7 (HIT2) (eBioscience), CD93-PE or Biotin (R3) (eBioscience), CD45-APC or FITC (2D1 or H130) (eBioscience and BD Bioscience), CD3-PE-Cy5 (UCHT1) (BD Biosciences) and CD64-PE (10.1) (BioLegend). Analyses and cell sorting experiments were performed using a FACS Vantage or FACS Aria (BD Biosciences) equipped with Diva software (BD), and data were analyzed using FlowJo (Tree Star).

Iwasaki M., Liedtke M., Gentles A.J, & Cleary M.L. (2015). CD93 marks a non-quiescent human leukemia stem cell population and is required for development of MLL-rearranged acute myeloid leukemia. Cell stem cell, 17(4), 412-421.

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