Synergi 4 u hydro rp 80a 250 3.0 mm

Chromatographic analyses were performed with the DIONEX Ultimate 3000 HPLC system coupled with a chromatographic column (Phenomenex Synergi 4 u Hydro-RP 80A 250_3.0 mm) set at 40 °C and a flow rate of 0.9 mL/min. Gradients of mobile phases (mobile phase A: 0.1% formic acid in water and mobile phase B: 0.1% formic acid in acetonitrile) were performed for a total of 25 min. MS analysis was carried out on a Thermo Scientific Exactive Plus Benchtop Orbitrap mass spectrometer (Thermo Fischer Scientific Inc., Waltham, MA, USA). The heated electrospray ionization source (HESI II) was used in positive- and negative-ion modes. The instrument was operated in full scan mode from m/z 67 to m/z 1000. A quality-control sample was prepared from an equal mixture of ten benign samples and used to monitor the stability of the mass spectrometer performance.

Chromatographic analysis was performed with the DIONEX Ultimate 3000 HPLC system coupled to a chromatographic column (Phenomenex Synergi 4 u Hydro-RP 80A 250_3.0 mm) set at 40 °C and a flow rate of 0.9 mL/min. Gradients of mobile phases (mobile phase A: 0.1% formic acid in water and mobile phase B: 0.1% formic acid in acetonitrile) were performed over a total of 25 min. MS analysis was carried out on a Thermo Scientific Exactive Plus Benchtop Orbitrap mass spectrometer. The heated electrospray ionization source (HESI II) was used in positive and negative ion modes. The instrument was operated in full scan mode from m/z 67 to m/z 1000. Post-treatment of data was performed using the MZmine2 version 2.53 (http://mzmine.github.io/) [22 (link)]. Metabolites were identified using the Metlin database (https://metlin.scripps.edu/).