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S6050

Manufactured by R&D Systems
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The S6050 is a laboratory instrument designed for various research and development applications. It features precise temperature control and monitoring capabilities to support experimental protocols that require stable thermal environments. The core function of the S6050 is to maintain and regulate the temperature of samples or materials under investigation.

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Lab products found in correlation

Slb50, by R&D Systems (2 mentions) Sta00c, by R&D Systems (2 mentions) Mrp300, by R&D Systems (1 mentions) Smta00b, by R&D Systems (1 mentions) Sm6000b, by R&D Systems (1 mentions) Cobas mira analyzer, by Roche (1 mentions)

3 protocols using s6050

1

Quantifying Cytokine Levels by ELISA

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Human serum and PBMC supernatant cytokine content was measured by ELISA (R&D: SLB50, STA00C and S6050) according to the manufacturer’s instructions. The measurements were performed in technical duplicates. Student’s t-test was performed for the statistical analysis. Mouse serum and BMDMs supernatant cytokine content was measured by ELISA (eBioscience for TNF and IL-6 and R&D for IL-1β) according to the manufacturer’s instructions.
Lalaoui N., Boyden S.E., Oda H., Wood G.M., Stone D.L., Chau D., Liu L., Stoffels M., Kratina T., Lawlor K.E., Zaal K.J., Hoffmann P.M., Etemadi N., Shield-Artin K., Biben C., Tsai W.L., Blake M.D., Kuehn H.S., Yang D., Anderton H., Silke N., Wachsmuth L., Zheng L., Moura N.S., Beck D.B., Gutierrez-Cruz G., Ombrello A.K., Pinto-Patarroyo G.P., Kueh A.J., Herold M.J., Hall C., Wang H., Chae J.J., Dmitrieva N.I., McKenzie M., Light A., Barham B.K., Jones A., Romeo T.M., Zhou Q., Aksentijevich I., Mullikin J.C., Gross A.J., Shum A.K., Hawkins E.D., Masters S.L., Lenardo M.J., Boehm M., Rosenzweig S.D., Pasparakis M., Voss A.K., Gadina M., Kastner D.L, & Silke J. (2019). Mutations that prevent caspase cleavage of RIPK1 cause autoinflammatory disease. Nature, 577(7788), 103-108.
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2

Quantifying Cytokine Levels by ELISA

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Human serum and PBMC supernatant cytokine content was measured by ELISA (R&D: SLB50, STA00C and S6050) according to the manufacturer’s instructions. The measurements were performed in technical duplicates. Student’s t-test was performed for the statistical analysis. Mouse serum and BMDMs supernatant cytokine content was measured by ELISA (eBioscience for TNF and IL-6 and R&D for IL-1β) according to the manufacturer’s instructions.
Lalaoui N., Boyden S.E., Oda H., Wood G.M., Stone D.L., Chau D., Liu L., Stoffels M., Kratina T., Lawlor K.E., Zaal K.J., Hoffmann P.M., Etemadi N., Shield-Artin K., Biben C., Tsai W.L., Blake M.D., Kuehn H.S., Yang D., Anderton H., Silke N., Wachsmuth L., Zheng L., Moura N.S., Beck D.B., Gutierrez-Cruz G., Ombrello A.K., Pinto-Patarroyo G.P., Kueh A.J., Herold M.J., Hall C., Wang H., Chae J.J., Dmitrieva N.I., McKenzie M., Light A., Barham B.K., Jones A., Romeo T.M., Zhou Q., Aksentijevich I., Mullikin J.C., Gross A.J., Shum A.K., Hawkins E.D., Masters S.L., Lenardo M.J., Boehm M., Rosenzweig S.D., Pasparakis M., Voss A.K., Gadina M., Kastner D.L, & Silke J. (2019). Mutations that prevent caspase cleavage of RIPK1 cause autoinflammatory disease. Nature, 577(7788), 103-108.
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3

Metabolic Assessments in Fasting Mice

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Mice were fasted overnight before metabolic analysis, and blood samples were drawn from the retro-orbital plexus of the fasting mice under anesthesia. We used ELISAs to determine serum levels of insulin (ALPCO; 80-INSMSU-E01), leptin (ALPCO; 22-LEPMS-E01), and adiponectin (R&D Systems; MRP300). We used a colorimetric/fluorometric kit to determine serum and tissue levels of NEFAs (BioVision; K612) and ELISA kits to determine serum and tissue levels of TNF-α (R&D Systems; SMTA00B) and IL-6 (R&D Systems; SM6000B and S6050). We measured serum cholesterol and TG levels with a Cobas Mira analyzer (Roche Diagnostics) at the Mouse Metabolic Phenotyping Center in Yale University.
For glucose tolerance tests (GTTs), we determined basal fasting glucose levels and then administered dextrose (1 g/kg body weight) by i.p. injection. For ITTs, we administered insulin (0.35 U/kg body weight for NCD-fed mice, 0.6 U/kg body weight for HFD-fed mice) by i.p. injection. We obtained blood samples from the tail vein 15, 30, 60, and 120 min after injection and determined blood glucose levels using a glucometer (LifeScan).
He F., Huang Y., Song Z., Zhou H.J., Zhang H., Perry R.J., Shulman G.I, & Min W. (2020). Mitophagy-mediated adipose inflammation contributes to type 2 diabetes with hepatic insulin resistance. The Journal of Experimental Medicine, 218(3), e20201416.
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