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Cd45.2 apc 104

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CD45.2-APC (104) is a fluorescently labeled antibody that binds to the CD45.2 antigen. It is a useful tool for the identification and analysis of mouse leukocytes.

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Lab products found in correlation

Fugene, by Promega (1 mentions) Facscalibur, by BD (1 mentions) Facs lysing solution, by BD (1 mentions) Cd19 pe 1d3, by BD (1 mentions) Heparinized tubes, by Greiner (1 mentions) Cd4 fitc gk1, by BD (1 mentions) Cd19 fitc 1d3, by BD (1 mentions)

Spelling variants of this product

CD45-APC (69 citations) CD45.2 (104, (13 citations) CD45.2-APC (11 citations) APC-Cy7 conjugated anti-CD45.2 (clone 104, (2 citations)

2 protocols using cd45.2 apc 104

1

Analyzing Ptprc Protein Expression in HEK293T Cells

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HEK293T cells were transfected (FuGENE, Promega) with pLEX_307 plasmids driving expression of the native or mutant mouse Ptprc protein described above. Four days following transfection, cells were trypsinized and the single-cell suspension was incubated with fluorochrome-conjugated antibodies CD45.1-FITC (A20) and CD45.2-APC (104) (eBioscience). The cells were analyzed on an LSR II instrument (BD Biosciences).
Mercier F.E., Sykes D.B, & Scadden D.T. (2016). Single Targeted Exon Mutation Creates a True Congenic Mouse for Competitive Hematopoietic Stem Cell Transplantation: The C57BL/6-CD45.1STEM Mouse. Stem Cell Reports, 6(6), 985-992.
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2

Immunophenotyping of Murine Peripheral Blood

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Peripheral blood samples were collected in heparinized tubes (Greiner Bio-One). In total, 50 μL of whole blood was incubated with 2% FCS and 2 mM EDTA in PBS containing the following staining antibodies: CD3-PerCP-eF710 (17A2, eBioscience), CD4-FITC (GK1.5, BD Biosciences), CD8-PE (53-6.7, BD Biosciences), CD19-FITC (1D3, BD Biosciences), CD19-PE (1D3, BD Biosciences), and CD45.2-APC (104, eBiosciences). For lysis of RBCs, 450 μL of 1× BD FACS Lysing Solution (BD Biosciences) was added. Samples were analyzed using a BD FACSCalibur (BD Biosciences) and FlowJo software. Graded numbers of ProCOUNT counting beads (BD Biosciences) were added to the blood samples to enumerate absolute cell numbers per μL. The gating strategy is described in Supplemental Figure 9.
Seefried M., Hundhausen N., Kroeger I., Büttner-Herold M., Hoffmann P., Edinger M., Ullrich E., Berberich-Siebelt F., Britt W.J., Mach M, & Winkler T.H. (2023). Murine cytomegalovirus reactivation concomitant with acute graft-versus-host disease is controlled by antibodies. JCI Insight, 8(5), e149648.
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