Nile blue dye

Manufactured by Merck Group

Sourced in Ireland

Nile Blue Dye is a synthetic dye commonly used in laboratory applications. It is a blue-green, water-soluble compound that is used as a staining agent for various biological and chemical procedures. The core function of Nile Blue Dye is to provide a colorimetric indicator in analytical techniques, such as fluorescence microscopy and protein electrophoresis.

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Lab products found in correlation

3 protocols using nile blue dye

Osteogenic Differentiation of SaOS2 Cells

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Polymethylmethacrylate (PMMA M_v = 120,000, Cat No: 182230), Poly (2-hydroxyethly methacrylate (PHEMA M_v = 300,000, Cat No: 192066), tetrahydrofuran (THF, Cat No: 401757), dimethylformamide (DMF, Cat No: D4551), glycerol-2-phosphate sodium salt (Cat No: G9422), ascorbic acid (Cat No: 1043003), Nile Blue Dye (Cat No: N5632) was purchased from Sigma-Aldrich (St Louis, MO). Human SaOS2 cells (Cat No: HTB85) and McKoy’s 5 A Media (Cat No: 30–2007) was purchased from ATCC (Manassas, VA), Heat treated Fetal Bovine Serum was purchased from Atlanta Biologicals (Lawrence, GA) (Cat No: S11050). Monoclonal mouse anti-human RUNX2 antibody (Cat No: sc-390351) was from Santa Cruz Biotechnology (Dallas, TX). In cell western (ICW) reagents; Odyssey Blocking buffer (Cat No: 92750000), LICOR IRDye 800 CW goat anti-mouse secondary antibody (Cat No: 925–32210), LICOR IRDye 680 RD goat anti-mouse secondary antibody (Cat No: 925–68071) were purchased from LICOR (Lincoln, NE).

Ozdemir T., Bowers D.T., Zhan X., Ghosh D, & Brown J.L. (2019). Identification of Key Signaling Pathways Orchestrating Substrate Topography Directed Osteogenic Differentiation Through High-Throughput siRNA Screening. Scientific Reports, 9, 1001.

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Confocal Microscopy Analysis of Emulsions

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Microstructural analysis of emulsions was performed using a Leica TCS SP Confocal Laser Scanning Microscope (Leica Microsystems, Heidelberg GmbH, Mannheim, Germany). Protein and lipid were fluorescently labelled with Nile Blue dye (Sigma-Aldrich, Dublin, Ireland). For the preparation of samples, 1 mL of the emulsion was mixed with 4 mL of low gelling temperature agarose (Sigma-Aldrich, St. Louis, MO, United States) solution (1.5%, w/v) at 30 °C, in order to prevent the movement of the oil globules during the analysis. Afterwards, 1 mL of the mixture was added to 50 µL of Nile Blue and incubated at 22 °C until the sample was solid. Visualisation of oil and protein in emulsions was carried out using an Ar laser (excitation 488 nm, emission 520–620 nm) and a He-Ne laser (excitation 633 nm, emission 650–730 nm) for oil (green) and protein (red), respectively. The observations were performed using 100× oil immersion objectives.

Alonso-Miravalles L., Zannini E., Bez J., Arendt E.K, & O’Mahony J.A. (2020). Thermal and Mineral Sensitivity of Oil-in-Water Emulsions Stabilised using Lentil Proteins. Foods, 9(4), 453.

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Synthesis and Characterization of Graphene Oxide

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The chemicals
and reagents used in this work include graphite (Sigma-Aldrich, 99%),
sodium nitrate (Sigma-Aldrich, 99.99%), sulfuric acid (Sigma-Aldrich,
95–97%), potassium per magnate (Sigma-Aldrich, ≥ 99.0%),
hydrogen peroxide (Sigma-Aldrich, 30% (w/w) in H₂O), silver
nitrate (Sigma-Aldrich, 99.99%), hydrazine hydrate (Sigma-Aldrich,
24–26%), sodium hydroxide (Sigma-Aldrich, reagent grade, 97%),
fuming hydrochloric acid (Sigma-Aldrich, 37%), ethanol (Sigma-Aldrich,absolute,
≥ 99.8%), nitric acid (Sigma-Aldrich, 70%), ammonia (Sigma-Aldrich,
anhydrous, ≥ 99.95%), and Nile blue dye (Sigma-Aldrich, 95%).
Deionized water was used throughout experiments. All the chemicals
were of high purity and purchased from Sigma-Aldrich supplier. The
equipment used in this project include digital balance, hot plate
with magnetic stirrer, drying oven, digital orbital shaker, Teflon-lined
autoclave, centrifuge (Eppendorf, Model 5804R), pH meter (Thermo Fisher),
UV–C light, ultraviolet–visible (UV–vis) spectrophotometer(PerkinElmer,
Lambda 35), X-ray diffraction (XRD, Bruker-D8 Advance), scanning electron
microscopy (SEM, JEOL-JSM-7001F), energy-dispersive X-rays (EDX, JEOL),
and Fourier transform infrared (FTIR, PerkinElmer-100 FT-IR, USA)
spectrometer.

N.a.t.a.s.h.a., Khan A., Rahman U.U., S.a.d.a.f., Yaseen M., Abumousa R.A., Khattak R., Rehman N., Bououdina M, & Humayun M. (2024). Effective Removal of Nile Blue Dye from Wastewater using Silver-Decorated Reduced Graphene Oxide. ACS Omega, 9(17), 19461-19480.

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