Human bronchial epithelial (HBE) cells and human fetal lung fibroblast MRC-5 cells were purchased from Procell (Wuhan, China) and cultured in DMEM supplemented with 10% fetal bovine serum and 1% streptomycin/penicillin mixture. When reaching about 80% confluence, cells were passaged with 0.25% trypsin digestion, and plated in 12-well plates and cultured overnight. Subsequently, the medium was changed to fresh culture medium with 10% fetal bovine serum, and subjected to different treatments. A humidified 5% CO2 atmosphere was used to maintain all cell cultures at 37°C.
Mrc 5 cells
Manufactured by Procell
Sourced in China
MRC-5 cells are a type of human diploid fibroblast cell line derived from the lung tissue of a 14-week-old male fetus. These cells are commonly used in various areas of research and biomedical applications.
Automatically generated - may contain errors
Lab products found in correlation
3 methyladenine 3 ma, by MedChemExpress (1 mentions)
H2170 cells, by Procell (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)
Ham s f 12k, by Thermo Fisher Scientific (1 mentions)
A549 cells, by Procell (1 mentions)
Hacat cells, by CLS Cell Lines Service (1 mentions)
4 protocols using mrc 5 cells
1
Culturing Human Lung Cell Lines
2
Herbal Medicines Modulate Fibroblast Autophagy
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The MRC-5 cells were purchased from Wuhan Procell Life Technology Co., Ltd. (Wuhan, China) and cultured in minimum essential medium (MEM) containing 10% fetal bovine serum and 1% penicillin and streptomycin at 37 ℃. The cells were divided into five groups: a control group, TGF-β1 group, qi-invigorating (AR) group, blood-activation (AS) group, and qi-invigorating and blood-activation (AR + AS) group. MRC-5 cells at 5×104 cells/well were pretreated with 100 µg/mL AR, AS, or AR + AS for 1 h, and then TGF-β1 (5 ng/mL) was added to each well for 24 h. MRC-5 cells were treated with 3-methyladenine (3-MA; 1 mM; MedChemExpress, Monmouth Junction, NJ, USA) for 1 h prior to TGF-β1 treatment.
3
Cultivation of Lung Cell Lines
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Lung squamous cell carcinoma cell line (H2170 cells) (product number: CL-0394), lung adenocarcinoma cell line (A549 cells) (product number: CL-0016), and human embryonic lung cell line (MRC-5 cells) (product number: CL-0161) were obtained from Procell (Wuhan, China) and orderly maintained in Roswell Park MEMorial Institute (RPMI)-1640 medium (Gibco, Grand Island, NY, USA), Ham’s F-12K (Thermo Fisher Scientific, Waltham, MA, USA), or Minimum Essential Medium (MEM) (Thermo Fisher Scientific) plus 10% fetal bovine serum (FBS) (Gibco) and 1% penicillin/streptomycin (Gibco) at 37°C and 5% CO2. The cell culture medium was replaced when cells were cultured for 3–4 days.
4
Murine Melanoma Cell Line Comparison
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Human non-small cell lung cancer (NSCLC) A549 cells were purchased from National Infrastructure of Cell Line Resource (NSTI). Mouse melanoma B16-F10 cells were purchased from Hunan Fenghui Biotechnology Co., Ltd. Mouse melanoma B16-BL6 cells were kindly provided by Mukogawa Women's University, Japan. MRC-5 cells were purchased from Procell Life Science & Technology Co., Ltd. HaCaT cells were obtained from CLS Cell Lines Service.
There are multiple sublines of C57BL/6 mouse melanoma cells B16 with different metastatic potentials despite their same origin. B16-BL6 is more metastatic and aggressive than B16-F10. The low metastatic subline Bl6-F10 was established by intravenous injection of B16 melanoma cells in static lung lesions, whereas the metastatic B16-F10 cells were established by 10 successive selections for metastasis following iv injection. Highly metastatic B16-BL6 cells were established by penetrating mouse bladder membranes with B16-F10 cells.
There are multiple sublines of C57BL/6 mouse melanoma cells B16 with different metastatic potentials despite their same origin. B16-BL6 is more metastatic and aggressive than B16-F10. The low metastatic subline Bl6-F10 was established by intravenous injection of B16 melanoma cells in static lung lesions, whereas the metastatic B16-F10 cells were established by 10 successive selections for metastasis following iv injection. Highly metastatic B16-BL6 cells were established by penetrating mouse bladder membranes with B16-F10 cells.
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