Hepes and L-arginine monohydrochloride were purchased from “Sigma-Aldrich” (St. Louis, MO, USA), 1,4-dithiothreitol (DTT) was purchased from PanReac (Barcelona, Spain), NaCl was purchased from “Reakhim” (Moscow, Russia). The procedure of isolation of Phb from rabbit skeletal muscles was described in [54 (link)].
1 4 dithiothreitol
Manufactured by ITW Reagents
Sourced in United States, Spain
1,4-Dithiothreitol is a reducing agent commonly used in biochemical applications. It is a small organic molecule that acts as a mild reducing agent, capable of breaking disulfide bonds in proteins. This property makes it useful for maintaining proteins in their reduced state during various experimental procedures.
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Lab products found in correlation
2 protocols using 1 4 dithiothreitol
1
Isolation of Phb from Rabbit Muscles
2
Purification of Bovine Liver GDH
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Bovine liver GDH (suspension in ammonium sulfate), hen egg white lysozyme, mono- and dibasic sodium phosphates, sodium chloride, and polyethylene glycol with a molecular mass of 20,000 Da (PEG) were purchased from Sigma-Aldrich (St. Louis, MO, USA). 1,4-dithiothreitol (DTT) was purchased from PanReac (Barcelona, Spain). Betaine was purchased from ICN Biomedicals (Costa Mesa, CA, USA). All solutions for the experiments were prepared using deionized water obtained with the Arium Mini system (Sartorius, Göttingen, Germany) and Simplicity UV system (Millipore, Burlington, MA, USA).
All samples and stock solutions were prepared in 0.1 M Na-phosphate buffer, pH 7.6, and 10 mM NaCl.
All samples and stock solutions were prepared in 0.1 M Na-phosphate buffer, pH 7.6, and 10 mM NaCl.
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