Alexafluor 680 conjugated antibodies

Manufactured by Thermo Fisher Scientific

AlexaFluor-680 conjugated antibodies are a type of fluorescent-labeled antibody. They are designed for use in various molecular biology and immunology applications that require sensitive detection of target proteins or other biomolecules. The AlexaFluor-680 fluorescent dye is attached to the antibody, allowing for visualization and quantification of the targeted analyte.

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Lab products found in correlation

Alexa fluor 488 conjugated antibody, by Thermo Fisher Scientific (2 mentions) Alexafluor 568 conjugated antibodies, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

AlexaFluor 680- conjugated secondary antibodies (3 citations) AlexaFluor 680-or −800 conjugated anti-rabbit and anti-mouse secondary antibodies (2 citations)

2 protocols using alexafluor 680 conjugated antibodies

Antibody-based Detection for Cell Signaling

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For Western blot analysis, specific primary antibodies against RanBP1 and HURP were purchased from Cell Signaling (#8780) and Bethyl (A300-853A) respectively. Antibodies against RCC1 and RanGAP1 were produced in rabbits using full-length recombinant RCC1 and RanGAP1 as antigens. Secondary HRP-conjugated anti-rabbit antibodies were purchased from Sigma-Aldrich. For immunostaining analysis, anti-mouse (A28175) and anti-rabbit (A11034) AlexaFluor-488 conjugated antibodies (Invitrogen); anti-mouse (A11004) and anti-rabbit (A11011) AlexaFluor-568 conjugated antibodies (Invitrogen); anti-mouse (A27042) AlexaFluor-680 conjugated antibodies (Invitrogen) were used.

Yau K.C., Arnaoutov A., Aksenova V., Kaufhold R., Chen S, & Dasso M. (2020). RanBP1 controls the Ran pathway in mammalian cells through regulation of mitotic RCC1 dynamics. Cell Cycle, 19(15), 1899-1916.

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Antibody Validation for Protein Analysis

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The following antibodies were used for immunostaining and western blot analysis: anti-mouse (A28175) and anti-rabbit (A11034) AlexaFluor-488-conjugated antibodies (Invitrogen), anti-mouse (A11004) and anti-rabbit (A11011) AlexaFluor-568-conjugated antibodies (Invitrogen), anti-mouse (A27042) AlexaFluor-680-conjugated antibodies (Invitrogen), and anti-mouse (A-31553) AlexaFluor-405-conjugated antibodies. Specific primary antibodies against NUP50 (A301-782A), NUP153 (A301-789A), TPR (A300-828A, A301-827A), GANP (A303-128A for WB; A303-127A for IF), NXF1 (A303-913A for IF; A303-915A for WB), NUP133 (A302-386A), NUP98 (sc-30112), HA (11867423001), FLAG M2 (F1804), ACTB (13E5, 4970S), SON (GTX129778), and Mab414 (MMS-120P) were purchased from Bethyl Laboratories, Santa Cruz Biotechnology, Sigma-Aldrich, GeneTex, and BioLegend, respectively. Secondary horseradish peroxidase-conjugated anti-mouse and anti-rabbit antibodies for western blot analysis were purchased from Sigma-Aldrich.

Aksenova V., Smith A., Lee H., Bhat P., Esnault C., Chen S., Iben J., Kaufhold R., Yau K.C., Echeverria C., Fontoura B., Arnaoutov A, & Dasso M. (2020). Nucleoporin TPR is an integral component of the TREX-2 mRNA export pathway. Nature Communications, 11, 4577.

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