7t 30 cm aviii spectrometer

Rats were anesthetized via a nose cone with 1.5–2.5% isoflurane and O2. The rats were positioned on an animal bed with a stereotaxic head holder and placed in the scanner. A rectal temperature probe was used for monitoring and maintenance of temperature at 37.0 ± 0.5°C using a warm air heating system. Respiration was monitored (SA Instruments, Stonybrook, New York). MRI was performed using a 7T/30-cm AVIII spectrometer (Bruker Biospin, Billerica, Massachusetts) equipped with a 12 cm gradient set and using an 86 mm quadrature RF transmit volume coil, a 2-channel receive surface RF coil and Paravision 6.0.1. A T2-weighted RARE sequence was used to visualize the glioma in both axial and coronal orientations, with the following parameters (axial): repetition time (TR)/echo time (TE) = 5000/80 ms, field of view (FOV) = 35 × 35 mm, acquisition matrix = 192 × 192, 25 slices with a slice thickness of 1 mm, 2 averages, and a RARE factor = 8. The coronal images used the same parameters except 13 slices were used. Tumor volumes were calculated by manual segmentation in a blinded fashion using DSI Studio software (http://dsi-studio.labsolver.org/).

MRI was used to confirm tumor presence and subsequently calculate tumor volume. Mice were anesthetized via a nose cone with 1%–2% isoflurane and O₂, positioned on an animal bed, and then placed in the scanner. MRI was performed using a 7T/30-cm AVIII spectrometer (Bruker Biospin) equipped with a 12-cm gradient set, an actively decoupled 86-mm quadrature RF volume transmit coil, a 2-channel mouse brain receiver array, and Paravision 6.0.1 software. A T₂-weighted RARE sequence was used to visualize the tumor, with the following parameters: repetition time (TR)/echo time (TE) = 5,000/40 ms, field of view (FOV) of 20 × 20 mm, acquisition matrix of 128 × 128, 25 slices with a slice thickness of 0.5 mm, four averages, and a RARE factor of 8. DSI Studio (https://dsi-studio.labsolver.org, build: February 20, 2022) was used to manually draw regions of interest (ROI) and calculate the tumor volumes.