For apoptosis staining, 1 × 106 cells of each sample were stained with anti‐Annexin‐V (BD Biosciences, San Jose, CA, USA) and 7‐AAD (eBioscience, San Diego, CA, USA). For staining of CCR5 and CXCR4, 1 × 106 cells per sample were stained with anti‐human CCR5 PE‐conjugated antibody (R & D, Minneapolis, MN, USA) or PE anti‐human CD184 (BD Pharmingen, San Jose, CA, USA). Intracellular p‐Akt, p‐Stat5, and Bcl‐2 staining was performed with 1 × 106 cells, which had been starved overnight, according to the manufacturer’s protocol. Briefly, cells were fixed with Cytofix Buffer (BD Biosciences) at 37°C for 15 min. Afterward, cells were permeabilized for 30 min on ice using Perm Buffer III (BD Biosciences, San Jose, USA) followed by two washing steps and were stained with Alexa 647 anti‐p‐Akt (pS473) (BD Biosciences), Alexa 647 anti‐p‐Stat5 (pY694) (BD, Biosciences) for 1 h or with PE anti‐Bcl‐2 (BD Biosciences) for 30 min. All samples were analyzed on a Fortessa cytometer (BD Biosciences), and data analysis was performed using flowjo V10 software (FlowJo LLC, Ashland, OR, USA).
Pe anti bcl 2
Manufactured by BD
Sourced in United States
PE anti-Bcl-2 is a fluorescence-labeled antibody that binds to the Bcl-2 protein. Bcl-2 is a key regulator of apoptosis, or programmed cell death. This product can be used in flow cytometry and other applications to detect and quantify Bcl-2 expression in cells.
Automatically generated - may contain errors
Lab products found in correlation
Perm buffer 3, by BD (1 mentions)
7 aad, by Thermo Fisher Scientific (1 mentions)
Cytofix buffer, by BD (1 mentions)
Fortessa cytometer, by BD (1 mentions)
Anti annexin 5, by BD (1 mentions)
Anti cd4 apc cy7, by BD (1 mentions)
Percp cy5.5 anti cxcr5, by BD (1 mentions)
Anti cd19 fitc, by BD (1 mentions)
Anti cd3 fitc, by BD (1 mentions)
Anti pd 1 pe, by BD (1 mentions)
Anti foxp3 pe, by BD (1 mentions)
Anti cxcr3 apc, by BD (1 mentions)
Anti cd38 apc, by BD (1 mentions)
Pe anti cd138, by BD (1 mentions)
Foxp3 staining buffer set, by Thermo Fisher Scientific (1 mentions)
2 protocols using pe anti bcl 2
1
Multiparametric Flow Cytometric Analysis of Apoptosis and Cell Signaling
2
Multi-Dimensional Immune Cell Profiling
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Single-cell suspensions were stained for 30 min using the following fluorophore-conjugated antibodies: FITC anti-CD3 (BD Biosciences Cat# 555332, RRID:AB_395739), APC/Cy7 anti-CD4 (BD Biosciences Cat# 341095, RRID:AB_400218), Percp-Cy5.5 anti-CXCR5 (BD Biosciences Cat# 562781, RRID:AB_2313576), PE anti-PD-1 (BD Biosciences Cat# 560795, RRID:AB_2033989), PE/Cy7 anti-CCR6 (BD Biosciences Cat# 560620, RRID:AB_1727440), APC anti-CCR6 (BD Biosciences Cat# 560619, RRID:AB_1727439), PECy7 anti-CXCR3 (BD Biosciences Cat# 560831, RRID:AB_2033944), APC anti-CXCR3 (BD Biosciences Cat# 565223, RRID:AB_2687488), FITC anti-CD19 (BD Biosciences Cat# 555412, RRID:AB_395812), BV421 anti-CD27 (BioLegend Cat# 302823, RRID:AB_10900425), APC anti-CD38 (BD Biosciences Cat# 555462, RRID:AB_398599), PECy7 anti-IgD (BD Biosciences Cat# 555776, RRID:AB_396111), and PE anti-CD138 (BD Biosciences Cat# 552026, RRID:AB_394323). For intracellular staining, cells were permeabilized using the Foxp3 Staining Buffer Set (eBioscience), then stained with PECy7 anti-Ki67 (BD Biosciences Cat# 561283, Clone B56), PE anti-Foxp3 (BD Biosciences Cat# 560082, RRID:AB_1645509), and PE anti-Bcl2 (BD Biosciences Cat# 340576, RRID:AB_400061). All samples were analyzed with an LSRFortessa flow cytometer (Beckton Dickinson), and data were processed with FlowJo software, version 10 (TreeStar, Inc.).
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