17β estradiol

Manufactured by Carl Roth

Sourced in Germany

17β-estradiol is a steroid hormone that belongs to the class of estrogens. It is the predominant and most potent form of estrogen in the body. 17β-estradiol plays a crucial role in the regulation of the female reproductive system and has various physiological functions.

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Lab products found in correlation

2 protocols using 17β estradiol

Methyl-C Metabolism in Denitratisoma

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17β-estradiol, S-adenosyl-l-methionine (SAM), S-adenosyl-l-homocysteine (SAH), N⁵-methyltetrahydrofolate, tetrahydrofolate (THF), methylviologen, benzylviologen, methylcobalamin (MeCo), nicotinamidadenindinucleotide-phosphate (NADPH), l-homocysteine, l-serine, ATP (ATP), l-methionine, and methyl-¹³C-l-methionine were purchased from Carl Roth (Karlsruhe, Germany), Carbosynth (Compton Berkshire, UK), or Merck (Darmstadt, Germany). Other chemicals or reagents were of analytical or high-performance-liquid chromatographic (HPLC) grade. Denitratisoma oestradiolicum (DSM 16959) was obtained from the Deutsche Sammlung für Mikroorganismen und Zellkulturen (DSMZ) (Braunschweig, Germany). Escherichia Coli strain BL21(DE3) was purchased from New England BioLabs (Frankfurt am Main, Germany).

Jacoby C., Krull J., Andexer J., Jehmlich N., von Bergen M., Brüls T, & Boll M. (2020). Channeling C1 Metabolism toward S-Adenosylmethionine-Dependent Conversion of Estrogens to Androgens in Estrogen-Degrading Bacteria. mBio, 11(4), e01259-20.

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Pharmacological characterization of vascular responses

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Equilin, 17β-estradiol, phenylephrine, acetylcholine, indomethacin, L-NAME (N^ω-nitro-L-arginine methyl ester), and KCl were purchased from Sigma-Aldrich (St. Louis, MO, USA). Tetraethylammonium, glibenclamide, 4-amynopiridine, ouabain, (S)-(−)-Bay K 8644, U46619 (9,11-dideoxy-11α,9α-epoxymethanoprostaglandin F2α), ICI 182,780, KT 5720, KT 5823, SQ 22,536 (9-(tetrahydro-2-furanyl)-9H-purin-6-amine), and ODQ (1H-[1 (link)–2 (link),4 (link)]oxadiazolo [4,3-alpha]-quinoxalin-1-one) were obtained from Tocris Bioscience (Bristol, UK). Caffeine was purchased from Roth (Karlsruhe, Germany).
Equilin and 17β-estradiol were dissolved in 70% v/v ethanol with further dilution in distilled water before use. Working concentrations of ethanol in the bath were < 0.01% (v/v).

Filgueira F.P., Lobato N.S., Nascimento D.L., Ceravolo G.S., Giachini F.R., Lima V.V., Dantas A.P., Fortes Z.B., Webb R.C., Tostes R.C, & Carvalho M.H. (2018). Equilin displays similar endothelium-independent vasodilator potential to 17β-estradiol regardless of lower potential to inhibit calcium entry. Steroids, 141, 46-54.

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