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Ebio244f4

Manufactured by Thermo Fisher Scientific
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The EBio244F4 is a laboratory instrument designed for electrochemical analysis. It provides precise measurements of current, voltage, and other electrochemical parameters. The core function of this product is to enable researchers and scientists to conduct electrochemical experiments and characterizations in various applications.

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Lab products found in correlation

Countbright beads, by Thermo Fisher Scientific (1 mentions) Facscanto, by BD (1 mentions) Sh800, by Sony (1 mentions) Ter119, by BD (1 mentions) Pd l1 10f 9g2, by BioLegend (1 mentions)

3 protocols using ebio244f4

1

Murine 2B4 Expression and Apoptosis

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Murine 2B4 expression was assessed using clone eBio244F4 (eBioscience). CountBright™ Beads (Thermo Fisher) were used to determine absolute cell numbers. For ICCS, splenocytes were incubated with 30 ng/mL PMA and 400 ng/mL ionomycin in the presence of GolgiStop for 4 hours at 37 °C. Apoptotic cells were determined by Caspase3/7 staining (ThermoFisher).
Chen C.W., Mittal R., Klingensmith N.J., Burd E.M., Terhorst C., Martin G.S., Coopersmith C.M, & Ford M.L. (2017). 2B4-mediated coinhibition of CD4+ T cells underlies mortality in experimental sepsis. Journal of immunology (Baltimore, Md. : 1950), 199(6), 1961-1966.
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2

SLAMF4 Antibodies for Mouse Studies

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Clones of SLAMF4 antibodies, B6 Alloantigen (BD Biosciences) and eBio244F4 (eBioscience) for C57BL-6 mice, and C9.1 for BALB-c mice were used in this study. The experimental set-up is described in detail in the online supplementary methods.
Cabinian A., Sinsimer D., Tang M., Jang Y., Choi B., Laouar Y, & Laouar A. (2017). Gut symbiotic microbes imprint intestinal immune cells with the innate receptor SLAMF4 which contributes to gut immune protection against enteric pathogens. Gut, 67(5), 847-859.
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3

Mouse Tissue Single-Cell Flow Cytometry

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Single-cell suspensions obtained from mouse tissues were incubated with Fc receptor-blocking anti-CD16/CD32, stained with fluorescent-conjugated antibodies, and analyzed by flow cytometry using FACSCanto (BD Biosciences) and FlowJo software (Tree Star) or subjected to cell sorting using SH800 (Sony). Antibodies specific to the following antigens were used in flow cytometry: CD71 (C2/C2F2; BD Biosciences), TER119 (TER-119; BD Biosciences), PDL1 (10F.9G2; BioLegend), and 2B4 (eBio244F4; eBioscience).
Sano Y., Yoshida T., Choo M.K., Jiménez-Andrade Y., Hill K.R., Georgopoulos K, & Park J.M. (2020). Multi-organ signaling mobilizes tumor-associated erythroid cells expressing immune checkpoint molecules. Molecular cancer research : MCR, 19(3), 507-515.
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