Secondary antibody goat anti rabbit

Manufactured by Huabio

The secondary antibody goat anti-rabbit is a laboratory reagent used in various immunoassay techniques, such as Western blotting, ELISA, and immunohistochemistry. It is a polyclonal antibody produced in goats and specifically recognizes and binds to rabbit primary antibodies, allowing for the detection and visualization of target proteins or antigens in a sample.

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Lab products found in correlation

Fkbp4, by Proteintech (2 mentions) Secondary antibody goat anti mouse, by Huabio (2 mentions) Flag tag (flag), by Merck Group (2 mentions) Naringenin, by Apexbio (1 mentions) Gapdh, by Proteintech (1 mentions) Tmem173, by Proteintech (1 mentions)

2 protocols using secondary antibody goat anti rabbit

Naringenin Treatment of Breast Cancer Cells

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Antibodies were used in the following dilutions: NRF2 (1:1,000, Proteintech, #16396-1-AP), FKBP4 (1:1,000, Proteintech, #10655-1-AP), NR3C1 (1:1,000, Proteintech, #24050-1-AP), P62 (1:1,000, MBL, #PM045), Histone (1:1,000, CST, #3638), GAPDH (1:1,000, Proteintech, #60004-1-Ig), Flag (1:1,000, Sigma, #F3165), HA (1:1,000, Biolegend, #901514), Secondary antibody goat anti-mouse (1:2,500, HuaBio, #HA1006), secondary antibody goat anti-rabbit (1:2,500, HuaBio, #HA1001), Anti-RABBIT IgG (H&L) (GOAT) Antibody Rhodamine Conjugated (1:200, MULTISCIENCES, #RK-611-1002), Anti-RABBIT IgG (H&L) (GOAT) Antibody ATTO 488 Conjugated (1:200, MULTISCIENCES, #RK-611-152-122S), Anti-Mouse CD11c, PE (1:20, MULTISCIENCES, #AM011C04), Anti-Mouse CD86 (B7-2), APC (AM08605). Naringenin was purchased from APExBIO (#N1370), and resolved in DMSO at 10 mM. BC cells were treated by medium with Naringenin for different points in time.

Xiong H., Chen Z., Lin B., Xie B., Liu X., Chen C., Li Z., Jia Y., Wu Z., Yang M., Jia Y., Wang L., Zhou J, & Meng X. (2022). Naringenin Regulates FKBP4/NR3C1/NRF2 Axis in Autophagy and Proliferation of Breast Cancer and Differentiation and Maturation of Dendritic Cell. Frontiers in Immunology, 12, 745111.

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Antibody Dilution Optimization Protocol

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Antibodies were used in the following dilutions: TMEM173 (1:1,000, Proteintech, #19851-1-AP), FKBP4 (1:1,000, Proteintech, #10655-1-AP), NR3C1 (1:1,000, Proteintech, #24050-1-AP), Beclin1 (1:1,000, CST, #3495S), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:1,000, Proteintech, #60004-1-Ig), Flag (1:1,000, Sigma, #F3165), hemagglutinin (HA) (1:1,000, Biolegend, #901514), secondary antibody goat anti-mouse (1:2,500, HuaBio, #HA1006), and secondary antibody goat anti-rabbit (1:2,500, HuaBio, #HA1001).

Xiong H., Chen Z., Lin B., Chen W., Li Q., Li Y., Fang M., Wang Y., Zhang H., Lu Y., Bi A., Wu S., Jia Y, & Wang X. (2022). Comprehensive analysis of FKBP4/NR3C1/TMEM173 signaling pathway in triple-negative breast cancer cell and dendritic cell among tumor microenvironment. Molecular Therapy Oncolytics, 24, 371-384.

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